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首页> 外文期刊>Transgenic research >Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases
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Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases

机译：高羊茅中噬菌体T4溶菌酶基因的表达赋予对灰斑病和棕斑病的抗性

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摘要

Tall fescue (Festuca arundinacea Schreb.) is an important turf and forage grass species worldwide. Fungal diseases present a major limitation in the maintenance of tall fescue lawns, landscapes, and forage fields. Two severe fungal diseases of tall fescue are brown patch, caused by Rhizoctonia solani, and gray leaf spot, caused by Magnaporthe grisea. These diseases are often major problems of other turfgrass species as well. In efforts to obtain tall fescue plants resistant to these diseases, we introduced the bacteriophage T4 lysozyme gene into tall fescue through Agrobacterium-mediated genetic transformation. In replicated experiments under controlled environments conducive to disease development, 6 of 13 transgenic events showed high resistance to inoculation of a mixture of two M. grisea isolates from tall fescue. Three of these six resistant plants also displayed significant resistance to an R. solani isolate from tall fescue. Thus, we have demonstrated that the bacteriophage T4 lysozyme gene confers resistance to both gray leaf spot and brown patch diseases in transgenic tall fescue plants. The gene may have wide applications in engineered fungal disease resistance in various crops.
机译：高羊茅（Festuca arundinacea Schreb。）是世界范围内重要的草皮和牧草种。真菌疾病在高羊茅草坪，风景和牧草场的维护方面存在主要限制。高羊茅的两种严重真菌病是由茄状枯萎病引起的棕色斑块和由稻瘟病引起的灰叶斑病。这些疾病通常也是其他草皮种类的主要问题。为了获得对这些疾病有抵抗力的高羊茅植物，我们通过农杆菌介导的遗传转化将噬菌体T4溶菌酶基因引入了高羊茅。在有利于疾病发展的受控环境下进行的重复实验中，在13个转基因事件中，有6个显示出对两种来自高羊茅菌的分离的稻瘟病菌混合物的高抗性。这六株抗性植物中的三株还显示出对高羊茅菌中的茄枯萎病菌的显着抗性。因此，我们证明了噬菌体T4溶菌酶基因在转基因高羊茅植物中赋予了对灰叶斑病和棕色斑块病的抗性。该基因可能在各种农作物的工程真菌抗病性中具有广泛的应用。

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