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首页> 外文期刊>Transgenic research >Overexpression of recombinant infectious bursal disease virus (IBDV) capsid protein VP2 in the middle silk gland of transgenic silkworm
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Overexpression of recombinant infectious bursal disease virus (IBDV) capsid protein VP2 in the middle silk gland of transgenic silkworm

机译：重组传染性法氏囊病病毒衣壳蛋白VP2在转基因家蚕中部过表达

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摘要

Infectious bursal disease virus (IBDV) is the causative agent of a highly contagious disease affecting young chickens and causes serious economic losses to the poultry industry worldwide. Development of subunit vaccine using its major caspid protein, VP2, is one of the promising strategies to protect against IBDV. This study aim to test the feasibility of using silkworm to produce recombinant VP2 protein (rVP2) derived from a very virulent strain of IBDV (vvIBDV). A total of 16 transgenic silkworm lines harboring a codon-optimized VP2 gene driven by the sericin1 promoter were generated and analyzed. The results showed that the rVP2 was synthesized in the middle silk gland of all lines and secreted into their cocoons. The content of rVP2 in the cocoon of each line was ranged from 0.07 to 16.10 % of the total soluble proteins. The rVP2 was purified from 30 g cocoon powders with a yield of 3.33 mg and a purity > 90 %. Further analysis indicated that the rVP2 was able to tolerate high temperatures up to 80 A degrees C, and exhibited specific immunogenic activity in mice. To our knowledge, this is the first report of overexpressing rVP2 in the middle silk gland of transgenic silkworm, which demonstrates the capability of silkworm as an efficient tool to produce recombinant immunogens for use in new vaccines against animal diseases
机译：传染性法氏囊病病毒（IBDV）是影响小鸡的高度传染性疾病的病原体，对全世界的家禽业造成严重的经济损失。利用其主要的壳蛋白VP2开发亚单位疫苗是预防IBDV的有前途的策略之一。这项研究的目的是测试使用家蚕生产源自极强力的IBDV株（vvIBDV）的重组VP2蛋白（rVP2）的可行性。总共生成并分析了由sericin1启动子驱动的，具有密码子优化的VP2基因的16个转基因蚕系。结果表明，rVP2在所有系的中间丝腺中合成并分泌到它们的茧中。每个品系的茧中rVP2的含量为可溶性蛋白总量的0.07％至16.10％。从30 g茧粉中纯化rVP2，产量为3.33 mg，纯度> 90％。进一步的分析表明，rVP2能够耐受高达80 A的高温，并在小鼠中表现出特定的免疫原活性。据我们所知，这是转基因蚕中部丝腺中rVP2过表达的第一份报道，证明了蚕作为生产重组免疫原用于动物疾病新疫苗的有效工具的能力。

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