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首页> 外文期刊>Transfusion and apheresis science: official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis >CD34+ cell content in unrelated allogeneic peripheral blood stem cell grafts transported internationally. Does the inter-laboratory variability affect comparability of graft quality data?
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CD34+ cell content in unrelated allogeneic peripheral blood stem cell grafts transported internationally. Does the inter-laboratory variability affect comparability of graft quality data?

机译：国际运输的无关的异体外周血干细胞移植物中的CD34 +细胞含量。实验室间的变异性是否会影响移植物质量数据的可比性？

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摘要

Background: Increasing numbers of unrelated hematopoietic stem cell grafts are transported internationally and evaluated concurrently in different laboratories. The graft quality assessment using the CD34+ enumeration could be influenced by inter-laboratory variability. Methods: We retrospectively analyzed the content of CD34+ cells in 154 consecutive collections being performed in different transplant centers during two periods (2003-2004, 2007-2010). All samples were tested twice in our own and partner laboratories. CD34+ percentage and absolute number were compared. Results: The percentage and the total CD34+ content correlated well in both observed periods (CD34+%: r=0.899 and r=0.922; CD34+×108/kg: r=0.966 and r=0.880; p0.0001). Median CD34+ percentages obtained in our centre in comparison with other laboratories were 0.54% vs. 0.46% in 2003-2004 and 0.69% vs. 0.70% in 2007-2010 period. The degree of laboratory compliance was affected by the laboratory identity. CD34+ percentage reported by one laboratory and CD34+×108/kg reported by three from twelve laboratories lacked statistically significant correlation with our own data. Conclusions: The study documented that results of CD34+ cell dose assessment of the same grafts reported by different transplant centers are comparable. The graft quality data and the CD34+ enumeration possess a limited level of inter-laboratory variability.
机译：背景：越来越多的无关的造血干细胞移植物被国际运输并在不同的实验室中同时进行评估。使用CD34 +枚举进行的移植物质量评估可能会受到实验室间变异性的影响。方法：我们回顾性分析了两个移植期（2003-2004年，2007-2010年）在不同移植中心进行的154个连续采集的CD34 +细胞的含量。所有样品均在我们自己的实验室和合作伙伴实验室中进行了两次测试。比较CD34 +百分比和绝对数。结果：在两个观察期中，CD34 +的百分比与总含量相关性很好（CD34 +％：r = 0.899和r = 0.922; CD34 +×108 / kg：r = 0.966和r = 0.880; p <0.0001）。与其他实验室相比，我们中心获得的CD34 +百分比中位数分别为0.54％和2003-2004年的0.46％和2007-2010年的0.69％和0.70％。实验室合规程度受实验室身份的影响。一个实验室报告的CD34 +百分比和十二个实验室的三个实验室报告的CD34 +×108 / kg与我们自己的数据缺乏统计学上的显着相关性。结论：该研究记录了不同移植中心报告的相同移植物的CD34 +细胞剂量评估结果具有可比性。移植物质量数据和CD34 +枚举在实验室间的变异程度有限。

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