Optimizing isolation culture and freezing methods to preserve Wharton's jelly's mesenchymal stem cell (MSC) properties: an MSC banking protocol validation for the Hellenic Cord Blood Bank

Chatzistamatiou Theofanis K.; Papassavas Andreas C.; Michalopoulos Efstathios; Gamaloutsos Christos; Mallis Panagiotis; Gontika Ioanna; Panagouli Effrosyni; Koussoulakos Stauros L.; Stavropoulos-Giokas Catherine

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Optimizing isolation culture and freezing methods to preserve Wharton's jelly's mesenchymal stem cell (MSC) properties: an MSC banking protocol validation for the Hellenic Cord Blood Bank

机译：优化隔离培养和冷冻方法以保留沃顿胶冻的间充质干细胞（MSC）特性：针对希腊脐带血库的MSC银行协议验证

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BackgroundMesenchymal stem or stromal cells (MSCs) are a heterogeneous population that can be isolated from many tissues including umbilical cord Wharton's jelly (UC-WJ). Although initially limited in studies such as a hematopoietic stem cell transplantation adjuvant, an increasing number of clinical trials consider MSCs as a potential anti-inflammatory or a regenerative medicine agent. It has been proposed that creating a repository of MSCs would increase their availability for clinical applications. The aim of this study was to assess the optimal isolation and cryopreservation procedures to facilitate WJ MSC banking.

机译：背景间充质干细胞或基质细胞（MSC）是一种异质种群，可以从许多组织中分离出来，包括脐带沃顿氏胶（UC-WJ）。尽管最初在诸如造血干细胞移植佐剂等研究中受到限制，但越来越多的临床试验认为MSCs是潜在的抗炎药或再生药物。已经提出，建立MSC的储存库将增加其在临床应用中的可用性。这项研究的目的是评估最佳分离和冷冻程序，以促进WJ MSC库。

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《Transfusion: The Journal of the American Association of Blood Banks》 |2014年第12期|共13页
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Chatzistamatiou Theofanis K.; Papassavas Andreas C.; Michalopoulos Efstathios; Gamaloutsos Christos; Mallis Panagiotis; Gontika Ioanna; Panagouli Effrosyni; Koussoulakos Stauros L.; Stavropoulos-Giokas Catherine;
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1. Optimizing isolation culture and freezing methods to preserve Wharton's jelly's mesenchymal stem cell (MSC) properties: an MSC banking protocol validation for the Hellenic Cord Blood Bank [J] . Chatzistamatiou Theofanis K., Papassavas Andreas C., Michalopoulos Efstathios, Transfusion: The Journal of the American Association of Blood Banks . 2014,第12期

机译：优化隔离培养和冷冻方法以保留沃顿胶冻的间充质干细胞（MSC）特性：针对希腊脐带血库的MSC银行协议验证
2. Angiogenic and osteogenic regeneration in rats via calcium phosphate scaffold and endothelial cell co-culture with human bone marrow mesenchymal stem cells (MSCs), human umbilical cord MSCs, human induced pluripotent stem cell-derived MSCs and human embryonic stem cell-derived MSCs [J] . Chen Wenchuan, Liu Xian, Chen Qianmin, Journal of tissue engineering and regenerative medicine . 2018,第1期

机译：大鼠血管生成和成骨再生通过磷酸钙支架和内皮细胞共同培养与人骨髓间充质干细胞（MSCs），人脐带MSCs，人诱导多能干细胞衍生的MSCs和人胚胎干细胞衍生的MSCs
3. Differentiation of human mesenchymal stem cells (MSC) to dopaminergic neurons: A comparison between Wharton's Jelly and olfactory mucosa as sources of MSCs [J] . Afizadeh Rafieh, Bagher Zohreh, Kamrava Seyed Kamran, Journal of chemical neuroanatomy . 2019,第期

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4. Neuromuscular regeneration using scaffolds with mesenchymal stem cells (MSCs) isolated from human umbilical cord Wharton's jelly: functional and morphological analysis using rat sciatic nerve neurotmesis injury model [C] . Ana Rita Caseiro, Tiago Pereira, Jorge Ribeiro International Conference on Tissue Engineering . 2015

机译：使用具有间充质干细胞（MSCs）的支架从人脐带涡轮果冻中分离的神经肌肉再生：使用大鼠坐骨神经神经性损伤模型的功能和形态学分析
5. Effects of Secretion Factors from Umbilical Cord Derived Mesenchymal Stem Cells (MSCs) on MSCs Multi-differentiation Potentials and Underlying Mechanisms [D] . Wang, Kuixing. 2014

机译：脐带间充质干细胞（MSCs）分泌因子对MSCs多分化潜能及其潜在机制的影响
6. Mesenchymal stem cells from the Wharton’s jelly of umbilical cord segments provide stromal support for the maintenance of cord blood hematopoietic stem cells during long-term ex vivo culture [O] . Tiki Bakhshi, Ryan C. Zabriskie, Shamanique Bodie, -1

机译：沃顿商学院脐带节胶质中的间充质干细胞在长期离体培养过程中为维持脐带血造血干细胞提供了基质支持
7. Co-Transplantation of Cord Blood with Wharton's Jelly Derived Umbilical Cord – Mesenchymal Stem Cell S (UC-MSC) Improves Short Term Myeloid Engraftment into NOD/SCID/IL-2Rγnull mice [O] . Paganessi L.A., Walker A.L., Fung H.C., 2009

机译：脐血与沃顿氏胶冻脐带-间充质干细胞S（UC-MSC）的共移植可改善NOD / SCID /IL-2Rγnull小鼠的短期骨髓植入

Optimizing isolation culture and freezing methods to preserve Wharton's jelly's mesenchymal stem cell (MSC) properties: an MSC banking protocol validation for the Hellenic Cord Blood Bank

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