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首页> 外文期刊>Transfusion: The Journal of the American Association of Blood Banks >Genomic typing of human red cell miltenberger glycophorins in a Taiwanese population.
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Genomic typing of human red cell miltenberger glycophorins in a Taiwanese population.

机译：台湾人口中人类红细胞miltenberger糖蛋白的基因组分型。

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摘要

BACKGROUND: Antigens in the human red cell Miltenberger series are glycophorin variants of the MN (MNS) blood group system that are due to the rearrangement of glycophorin A (GPA) and glycophorin B (GPB) genes. STUDY DESIGN AND METHODS: Taking advantage of the differences between the GPA and GPB genes, a polymerase chain reaction-based method was developed to detect all the Miltenberger glycophorin variants and St(a) subtype. GPA- and GPB-specific primers were used to amplify the GPA or GPB gene, and the amplified products were used to recognize the different hybrid genes after restriction enzyme digestions. RESULTS: Among 264 Taiwanese subjects studied, Mi.III and St(a) are the most common types of Miltenberger variants found. Mi.III was present in 13 (4.92%) of 264, and St(a) was found in 8 (3. 03%) of 264; 1 case (0.4%) of Mi.V was also identified from the study group. CONCLUSION: This is the first polymerase chain reaction-based method of detecting most of the Miltenberger variants and St(a). The genomic typing results were confirmed by control DNA of identified Miltenberger phenotypes. The prevalence rates of Mi. III and St(a) in this study were also consistent with other previous reports using different methods.
机译：背景：人类红细胞Miltenberger系列中的抗原是MN（MNS）血型系统的糖蛋白变体，这是由于糖蛋白A（GPA）和糖蛋白B（GPB）基因的重排所致。研究设计和方法：利用GPA和GPB基因之间的差异，开发了一种基于聚合酶链反应的方法来检测所有Miltenberger糖蛋白变体和St（a）亚型。 GPA和GPB特异性引物用于扩增GPA或GPB基因，扩增的产物用于在限制性酶消化后识别不同的杂种基因。结果：在研究的264名台湾受试者中，Mi.III和St（a）是发现的Miltenberger变体的最常见类型。在264的13（4.92％）中存在Mi.III，在264的8（3. 03％）中发现St（a）;还从研究组中鉴定出1例（0.4％）Mi.V。结论：这是第一种基于聚合酶链反应的方法，可检测大多数Miltenberger变体和St（a）。基因组分型结果由已鉴定的Miltenberger表型的对照DNA确认。 Mi的患病率。本研究中的III和St（a）也与以前使用不同方法的其他报道一致。

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