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首页> 外文期刊>Transfusion: The Journal of the American Association of Blood Banks >Platelet activation and residual activation potential during storage of hyperconcentrated platelet products in two different platelet additive solutions.
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Platelet activation and residual activation potential during storage of hyperconcentrated platelet products in two different platelet additive solutions.

机译：高浓度血小板产品在两种不同的血小板添加剂溶液中储存期间的血小板活化和残余活化潜能。

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BACKGROUND: To improve platelet (PLT) quality, hyperconcentrated PLT concentrates (hcPCs) were compared to standard PLT concentrates (stdPCs) in two different PLT additive solutions, T-Sol and PAS-27a. PAS-27a differs from T-Sol by containing glucose, phosphate, potassium, magnesium, and bicarbonate. STUDY DESIGN AND METHODS: PLTs were harvested by apheresis twice from 14 donors; each unit was divided into two. Four units from each donor were produced: hcPCs, 2000 x 10(9) per L in T-Sol or PAS-27a; and stdPCs, 1400 x 10(9) per L in 65 percent T-Sol or PAS-27a and 35 percent acid citrate dextrose-plasma. On Days 1 through 4, swirling was scored and PLT count, mean PLT volume, pH, blood gas, glucose, and lactate were measured. Expression of CD42a, CD62P, CD63, and PAC-1 was analyzed by flow cytometry on resting PLTs and PLTs stimulated with thrombin receptor agonist peptide (TRAP). RESULTS: Glucose consumption and lactate production were significantly higher in hcPCs stored in PAS-27a than in T-Sol. BothstdPC and hcPC PLTs in T-Sol expressed CD62P and PAC-1 significantly higher than in PAS-27a. Over time the T-Sol hcPCs revealed highest expression of CD62P and CD63. A significantly higher capacity for up regulation of CD62P, CD63, and PAC-1 upon TRAP stimulation was found for stdPCs and hcPCs in PAS-27a compared to PLTs in T-Sol. TRAP-stimulated PLTs in stdPCs and hcPCs suspended in PAS-27a showed significantly higher potential for down regulation of CD42a than the T-Sol concentrates. CONCLUSIONS: PLTs appear better preserved in vitro in PAS-27a than in T-Sol, and this suggests that storage of hcPCs in PAS-27a could be extended beyond 24 hours.
机译：背景：为了提高血小板（PLT）的质量，在两种不同的PLT添加剂溶液T-Sol和PAS-27a中将超浓缩PLT浓缩物（hcPCs）与标准PLT浓缩物（stdPCs）进行了比较。 PAS-27a与T-Sol的不同之处在于它包含葡萄糖，磷酸盐，钾，镁和碳酸氢盐。研究设计和方法：从14位供体中通过采血两次收集PLT。每个单元分为两个。每个供体产生四个单位：hcPCs，每L在T-Sol或PAS-27a中为2000 x 10（9）;和stdPC，在65％的T-Sol或PAS-27a和35％的柠檬酸右旋糖血浆中每升1400 x 10（9）。在第1至第4天，对漩涡进行评分，并测量PLT计数，平均PLT体积，pH，血气，葡萄糖和乳酸。通过流式细胞术分析了凝血酶受体激动剂肽（TRAP）刺激的静息PLT和PLT上CD42a，CD62P，CD63和PAC-1的表达。结果：储存在PAS-27a中的hcPC中的葡萄糖消耗和乳酸生成显着高于T-Sol。 T-Sol中的stdPC和hcPC PLTs均表达CD62P和PAC-1，明显高于PAS-27a。随着时间的流逝，T-Sol hcPCs显示出CD62P和CD63的最高表达。与T-Sol中的PLTs相比，发现PAS-27a中的stdPCs和hcPCs在TRAP刺激后具有上调CD62P，CD63和PAC-1的能力。悬浮在PAS-27a中的stdPCs和hcPCs中用TRAP刺激的PLTs比T-Sol浓缩物具有显着更高的CD42a下调潜能。结论：PAS-27a中PLTs的体外保存性优于T-Sol，这表明hcPCs在PAS-27a中的存储可以延长24小时以上。

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