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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Phenotypic and genotypic characterization of antibiotic-resistant soil and manure bacteria adjacent to swine production facilities
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Phenotypic and genotypic characterization of antibiotic-resistant soil and manure bacteria adjacent to swine production facilities

机译：猪生产设施附近的耐抗生素土壤和粪肥细菌的表型和基因型特征

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This study aimed to identify phenotypic and genotypic resistance profiles of bacteria isolated from soil and manure samples obtained from a farm where tetracycline and tylosin were used extensively. Samples were collected from the manure and soil, before and after manure application. All of the 151 bacteria were identified based on BLAST results. Minimum inhibitory concentrations were determined by agar dilution and E-test methods. Class-specific primers were used to amplify 6 erythromycin and 7 tetracycline resistance (Tc~r) genes for 46 tetracycline- and 18 erythromycin-resistant isolates. Tc~r gene sequence identity between different species of isolates was identified to investigate the dissemination of resistance genes. All of the 151 bacteria belonged to 4 different phyla: Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes. The majority of the tetracycline-resistant isolates were present in Pseudomonadaceae. Twenty of the isolates, representing 14 different genera, were positive forTc~r genes. Only 2 isolates possessed the erm(Q) gene. This study probably is the first to describe the presence of tetracycline genes in members of the genera Simplicispira and Agrococcus. Results of the study suggest that the diversity of Tc~r gene-carrying bacteria are increasing. Furthermore, tetracycline-resistant bacteria could be detected 3 months after the manure application.
机译：这项研究旨在确定从广泛使用四环素和泰乐菌素的农场获得的土壤和粪便样品中分离出的细菌的表型和基因型抗性谱。在施用肥料之前和之后，从肥料和土壤中收集样品。根据BLAST结果鉴定出所有151种细菌。最小抑菌浓度通过琼脂稀释和E-test方法确定。类特异性引物用于扩增46个四环素抗性和18个红霉素抗性分离株的6个红霉素和7个四环素抗性（Tc-r）基因。确定了不同菌种之间的Tc〜r基因序列同一性，以研究抗性基因的传播。所有151种细菌都属于4种不同的门：变形杆菌，拟杆菌，放线菌和Firmicutes。大部分对四环素具有抗性的分离物存在于假单胞菌科中。代表14个不同属的20个分离株对Tc〜r基因呈阳性。只有2个分离株具有erm（Q）基因。这项研究可能是第一个描述四环素基因存在于Simplicispira和Agrococcus属中的成员。研究结果表明，携带Tc〜r基因的细菌的多样性正在增加。此外，施肥3个月后可检测到四环素抗性细菌。

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