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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Detection of bovine tuberculosis by tuberculin test and polymerase chain reaction in Van, Turkey.
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Detection of bovine tuberculosis by tuberculin test and polymerase chain reaction in Van, Turkey.

机译：通过结核菌素试验和聚合酶链反应在土耳其范市检测牛结核病。

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摘要

The aim of this study was to identify the presence and prevalence of bovine tuberculosis in the eastern Anatolian border province of Van, Turkey and its villages and to collect the preliminary data required for programmes for the prevention and control of this zoonotic disease. The tuberculin test was performed on 210 cattle, and nasal swab and milk samples were obtained from the animals tested. Standardization of DNA extraction and amplification was performed using a Mycobacterium bovis strain obtained from the Tuberculosis Laboratories of the Veterinary Control and Research Institute of the Ministry of Agriculture and Rural Affairs. Three cattle that had a positive tuberculin test also tested positive for the DNA target, a 580-bp fragment of IS6110 specific for members of the Mycobacterium tuberculosis complex. This fragment was also recovered from milk taken from the third animal that tested positive for tuberculin. All the other cattle were negative for bovine tuberculosis under tuberculin test and PCR assay. The results of the tuberculin test and the PCR were in close correlation with each other. Van is a border province in eastern Anatolia. The prevalence of bovine tuberculosis was estimated to be 1.42% in the city centre and its villages.
机译：这项研究的目的是确定土耳其安那托利亚边境东部省范市及其村庄中牛结核病的存在和流行，并收集预防和控制这种人畜共患病的计划所需的初步数据。对210头牛进行了结核菌素测试，从被测动物中获得了鼻拭子和牛奶样品。 DNA提取和扩增的标准化是使用从农业和农村事务部兽医控制和研究所的结核病实验室获得的牛分枝杆菌菌株进行的。结核菌素试验阳性的三头牛也对DNA靶标呈阳性，这是结核分枝杆菌复合体成员特异性的IS6110的580 bp片段。该片段还从取自结核菌素检测呈阳性的第三只动物的牛奶中回收。在结核菌素试验和PCR分析下，所有其他牛的牛结核病均为阴性。结核菌素试验和PCR的结果彼此密切相关。范是安纳托利亚东部的边境省。市中心及其村庄的牛结核病患病率估计为1.42％。

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