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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Analysis of the ORF region of the prion protein gene in the Botosani Karakul sheep breed from Romania
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Analysis of the ORF region of the prion protein gene in the Botosani Karakul sheep breed from Romania

机译：罗马尼亚Botosani Karakul绵羊品种中the病毒蛋白基因ORF区域的分析

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摘要

Ovine scrapie is considered a neurodegenerative disease characterized by the accumulation of an abnormal, protease-resistant isoform of PrP. The polymorphisms at codons 136, 154, and 171 are associated with susceptibility to scrapie. In order to detect the existing polymorphisms at the 3 codons we used the real-time PCR technique. The obtained genotypes were confirmed by PCR amplification and sequencing of a 402 bp fragment of exon 3 of the prion gene. Known polymorphisms were detected at codons 136,154, and 171 and 5 different genotypes were determined. The results showed that all animals were homozygous for A at codon 136 and homozygous for R at codon 154 and 3 out of 4 previously identified polymorphisms at residue 171 were detected (R, H, and Q). Two new polymorphisms were detected at codon 189 and 199, as well as 2 other reported polymorphisms at codons 141 and 143. The frequency of high risk ARQ/ARQ genotype was about 47%. The aim of this study was to determine same preliminary information on the PrP gene-associated susceptibility in scrapie in the Botosani Karakul sheep breed.
机译：羊瘙痒病被认为是一种神经退行性疾病，其特征是PrP的异常，耐蛋白酶的同工型的积累。密码子136、154和171的多态性与瘙痒病的易感性有关。为了检测3个密码子存在的多态性，我们使用了实时PCR技术。通过对amplification病毒基因外显子3的402 bp片段进行PCR扩增和测序来确认获得的基因型。在密码子136,154处检测到已知的多态性，并确定了171和5种不同的基因型。结果表明，所有动物在136密码子处的A纯合，在R 154密码子处的R纯合，在先前发现的残基171的4种多态性中，有3种被检测到（R，H和Q）。在189和199密码子处检测到两个新的多态性，以及在141和143密码子处检测到两个其他的多态性。高风险ARQ / ARQ基因型的频率约为47％。这项研究的目的是确定有关Botosani Karakul绵羊品种的瘙痒病中PrP基因相关易感性的相同初步信息。

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