Cryotherapy of shoot tips: novel pathogen eradication method

Wang Q

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Cryotherapy of shoot tips: novel pathogen eradication method

机译：芽梢的冷冻疗法：新的病原体消灭方法

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Cryotherapy is a novel application of plant cryopreservation techniques that allows pathogen eradication at a high frequency. It eliminates plant pathogens such as viruses, phytoplasmas and bacteria by briefly treating shoot tips in liquid nitrogen using cryopreservation protocols. Healthy plants are regenerated from the surviving pathogen-free meristematic tissue. The method facilitates treatment of large numbers of samples and is independent of shoot tip size. It has the potential to replace more traditional methods like meristem culture.

机译：冷冻疗法是植物冷冻保存技术的一种新颖应用，可高频率根除病原体。通过使用冷冻保存方案短暂处理液氮中的茎尖，可消除植物病原体，例如病毒，植物原虫和细菌。从存活的无病原体的分生组织再生健康的植物。该方法有助于处理大量样品，并且与茎尖尺寸无关。它有可能替代更传统的方法，例如分生组织文化。

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《Trends in Plant Science》 |2009年第3期|共4页
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Wang Q;
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正文语种 eng
中图分类植物学;
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1. Cryotherapy of shoot tips: novel pathogen eradication method [J] . Wang Q Trends in Plant Science . 2009,第3期

机译：芽梢的冷冻疗法：新的病原体消灭方法
2. Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation [J] . Wang Q.C., Panis B., Engelmann F., Annals of Applied Biology . 2009,第3期

机译：芽梢的冷冻疗法：一种消灭病原体的技术，以生产健康的种植材料并准备健康的植物遗传资源进行冷冻保存
3. Eradication of latent viruses from apple cultivar `Monalisa' shoot tips using droplet-vitrification cryotherapy [J] . Bettoni Jean Carlos, Souza Juliana Aparecida, Volk Gayle M., Scientia horticulturae . 2019,第期

机译：使用液滴 - 玻璃化冷冻疗法消除苹果品种`Monalisa'拍摄提示的潜伏病毒
4. MANIPULATION OF COOLING RATE DURING CRYOPRESERVATION OF HYPERICUM PERFORATUM L. SHOOT TIPS USING SLOW FREEZING METHOD [C] . Matus SKYBA, Martina URBANOVA, Jan KOSUTH, . 2007

机译：冷冻法冷冻贯叶连翘冷冻过程中的冷却速率调节。
5. Cryopreservation of shoot tips: Antioxidant investigations with Rubus and protocols for Mentha and Vaccinium. [D] . Uchendu, Esther Eyiuche. 2009

机译：芽梢的冷冻保存：用Rubus进行的抗氧化研究以及薄荷和牛痘的操作规程。
6. Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution subcellular changes cell survival and viral RNA degradation in shoot tips [O] . QIAOCHUN WANG, WILMER J. CUELLAR, MINNA‐LIISA RAJAMÄKI, 2008

机译：热疗法和冷冻疗法相结合可有效消灭病毒：梢中病毒分布亚细胞变化细胞存活和病毒RNA降解的关系
7. Combining Thermotherapy with Cryotherapy for Efficient Eradication of Apple stem grooving virus from Infected In-vitro-cultured Apple Shoots [O] . Lei Zhao, Min-Rui Wang, Zhen-Hua Cui, 2018

机译：将热疗与冷冻疗法结合起来，从而有效地消除苹果茎的苹果芽感染的苹果茎槽病毒
8. Home Air Purifiers Eradicate Harmful Pathogens. [R] . 2014

机译：家用空气净化器根除有害病原体。

Cryotherapy of shoot tips: novel pathogen eradication method

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