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首页> 外文期刊>Trends in Plant Science >High-Resolution DNA Melting Analysis in Plant Research
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High-Resolution DNA Melting Analysis in Plant Research

机译:植物研究中的高分辨率DNA熔解分析

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摘要

Genetic and genomic studies provide valuable insight into the inheritance, structure, organization, and function of genes. The knowledge gained from the analysis of plant genes is beneficial to all aspects of plant research, including crop improvement. New methods and tools are continually being developed to facilitate rapid and accurate mapping, sequencing, and analyzing of genes. Here, I review the recent progress in the application of high-resolution melting (HRM) analysis of DNA, a method that allows detecting polymorphism in double stranded DNA by comparing profiles of melting curves. Use of HRM has expanded considerably in the past few years as the method was successfully applied for high-throughput genotyping, mapping genes, testing food products and seeds, and other areas of plant research.
机译:遗传和基因组研究为基因的遗传,结构,组织和功能提供了宝贵的见识。从植物基因分析中获得的知识有益于植物研究的各个方面,包括作物改良。不断开发新的方法和工具以促进快速,准确的基因定位,测序和分析。在这里,我回顾了DNA的高分辨率熔解(HRM)分析应用的最新进展,该方法允许通过比较熔解曲线的轮廓来检测双链DNA中的多态性。在过去的几年中,由于该方法已成功用于高通量基因分型,基因定位,食品和种子测试以及植物研究的其他领域,因此HRM的使用已大大扩展。

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