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首页> 外文期刊>Trends in Plant Science >The cohesion theory debate continues - Response by Herve Cochard, Thierry Ameglio and Pierre Cruiziat
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The cohesion theory debate continues - Response by Herve Cochard, Thierry Ameglio and Pierre Cruiziat

机译:凝聚力理论辩论仍在继续-Herve Cochard,Thierry Ameglio和Pierre Cruiziat的回应

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In two recent publications, we have reported that cryo-scanning electron microscopy (CSEM) observations of vessel content during transpiration in petioles of well-watered walnut trees were artifacts of the freezing procedure. We concluded that xylem vessels were all filled with sap during tree transpiration. Some of our experiments have been repeated recent]y3. Although the experiments yielded the same results, Martin Canny and colleagues came to the different conclusion that many vessels were filled with gas (embolized) during transpiration. We will summarize our experiment in this letter and explain where our views diverge. Canny and colleagues quantified vessel embolism by the proportion of vessels seen filled with gas on a cross section in a CSEM (Ref. 4). In our work, we have also measured the proportion of loss of hydraulic conductance5 (PLC) as a result of air embolism. The CSEM data suggest that many vessels are filled with gas in samples frozen intact on the tree but none in samples frozen shortly after excision under water. The PLC value of samples excised under water is also zero. Both techniques being invasive and, therefore, giving indirect information about the actual vessel content inplanta, the current debate is about the interpretation of these data. We have argued that the number of gas-filled vessels is abnormally high on samples frozen intact on the tree because cavitations (artifacts) occur during the freezing procedure.
机译:在最近的两篇出版物中,我们已经报道了在水分充足的核桃树的叶柄蒸腾过程中进行的冷冻扫描电子显微镜(CSEM)观测的容器含量是冷冻过程的产物。我们得出的结论是,木质蒸腾过程中木质部血管都充满了树液。最近,我们重复了一些实验。尽管实验得出的结果相同,但马丁·坎尼(Martin Canny)和同事得出了不同的结论,即在蒸腾过程中,许多血管充满了气体(栓塞)。我们将在这封信中总结我们的实验,并说明我们的观点存在分歧。 Canny及其同事通过在CSEM的横截面上看到的充满气体的血管比例来量化血管栓塞(参考文献4)。在我们的工作中,我们还测量了由于空气栓塞而造成的水力传导损失5(PLC)的比例。 CSEM数据表明,在完整冷冻在树上的样品中,许多容器中充满了气体,但在水下切除后不久后冷冻的样品中却没有气体。在水下切除的样品的PLC值也为零。两种技术都是侵入性的,因此,它们提供有关植物体内实际血管含量的间接信息,当前的争论是关于这些数据的解释。我们认为,在树上完整冷冻的样品上,充气容器的数量异常高,因为在冷冻过程中会发生气穴(伪像)。

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