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首页> 外文期刊>Trends in Plant Science >TREP: a database for Triticeae repetitive elements
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TREP: a database for Triticeae repetitive elements

机译：TREP：Triticeae重复元素的数据库

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Genomes of the important crop plants wheat, barley and rye are large (5 X 10~9 to 17 X 10~9 bp) and contain 80 percent repetitive sequences. Although research on the molecular genetics of Triticeae concentrates on gene-rich regions, most genomic and some EST sequences isolated from these species are repetitive. Rapid identification of repetitive elements could significantly speed up the process of gene discovery and chromosome walking. Unfortunately, wading through the quagmire of repetitive sequences can be a difficult and uninspiring task. Classification and naming of such elements has been somewhat arbitrary and occasionally the same elements have even been classified and named differently by different researchers (e.g. 'Sabrina' [1] and 'XA' [2]). Often, repetitive elements are not present as complete copies but are fragmented by the insertion of other elements or by deletions, adding to the complexity of the analysis. In addition, frequently, only PCR fragments of conserved domains in repetitive elements (e.g. sequences encoding reverse transcriptase) are available.
机译：重要农作物小麦，大麦和黑麦的基因组很大（5 X 10〜9至17 X 10〜9 bp），并包含80％的重复序列。尽管对小麦的分子遗传学研究集中在基因丰富的区域，但从这些物种分离的大多数基因组和一些EST序列是重复的。快速鉴定重复元件可以显着加快基因发现和染色体行走的过程。不幸的是，在重复序列的泥潭中涉水可能是一项困难而毫无启发的任务。此类元素的分类和命名在某种程度上是任意的，有时甚至由不同的研究人员对相同的元素进行分类和命名也不同（例如“ Sabrina” [1]和“ XA” [2]）。通常，重复元素不以完整副本的形式出现，而是由于其他元素的插入或删除而变得零碎，从而增加了分析的复杂性。另外，经常只有重复元件（例如编码逆转录酶的序列）中保守结构域的PCR片段可用。

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