RNA-Seq: revelation of the messengers.

Verk M. C. van; Hickman R.; Pieterse C. M. J.; Wees S. C. M. van

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RNA-Seq: revelation of the messengers.

机译：RNA-Seq：信使的启示。

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Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists. Here, we put forward the concepts and considerations that are critical for RNA-Seq data analysis and provide a generic tutorial with example data that outlines the whole pipeline from next-generation sequencing output to quantification of differential gene expression.

机译：下一代RNA测序（RNA-Seq）正在迅速超越微阵列，成为全转录组研究的首选技术。但是，RNA-Seq数据分析所需的生物信息学技能通常对许多生物学家构成重大障碍。在这里，我们提出了对于RNA-Seq数据分析至关重要的概念和注意事项，并提供了一个带有示例数据的通用教程，概述了从下一代测序输出到差异基因表达定量的整个流程。

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来源
《Trends in Plant Science》 |2013年第4期|共5页
作者
Verk M. C. van; Hickman R.; Pieterse C. M. J.; Wees S. C. M. van;
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原文格式 PDF
正文语种 eng
中图分类植物学;
关键词
bioinformatics; DNA sequencing; gene expression; genes; nucleotide sequences; transcriptomes; transcriptomics; DNA sequences; nucleotide sequence analysis; nucleotide sequencing;

机译：生物信息学;DNA测序;基因表达;基因;核苷酸序列;转录组;转录组学;DNA序列;核苷酸序列分析;核苷酸测序;

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RNA-Seq: revelation of the messengers.

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