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Probing plasma membrane dynamics at the single-molecule level

机译:在单分子水平上探测质膜动力学

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摘要

The plant plasma membrane is highly dynamic and,changes multiple aspects of its organization in response to environmental and internal factors. A detailed understanding of membrane dynamics in living plant cells has remained obscure because of the limited spatial resolution of conventional optical microscopy. Recently, several single-molecule imaging approaches have been developed and used to provide valuable insights into the fundamental biochemical and biophysical properties of the plant plasma membrane, including the organization of membrane microdomains and the dynamics of single-molecule diffusion. Here we review single-molecule imaging methods, including total internal reflection fluorescence microscopy (TIRFM), fluorescence correlation spectroscopy (FCS), and super-resolution microscopy, and examine their contributions to recent progress in understanding protein dynamics and membrane organization in living plant cells.
机译:植物质膜具有很高的动态性,可以根据环境和内部因素改变其组织的多个方面。由于常规光学显微镜的有限空间分辨率,对活植物细胞中膜动力学的详细了解仍然不清楚。最近,已经开发了几种单分子成像方法,用于为植物质膜的基本生化和生物物理特性提供有价值的见解,包括膜微域的组织和单分子扩散的动力学。在这里,我们回顾单分子成像方法,包括全内反射荧光显微镜(TIRFM),荧光相关光谱(FCS)和超分辨率显微镜,并检查它们对了解植物体内蛋白质动力学和膜组织的最新进展的贡献。 。

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