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首页> 外文期刊>Tumour biology : >Expression of serum let-7c, let-7i, and let-7f microRNA with its target gene, pepsinogen C, in gastric cancer and precancerous disease
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Expression of serum let-7c, let-7i, and let-7f microRNA with its target gene, pepsinogen C, in gastric cancer and precancerous disease

机译：血清let-7c，let-7i和let-7f及其靶基因胃蛋白酶原C在胃癌和癌前疾病中的表达

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摘要

This study examined the expression patterns of serum let-7 microRNA (miRNA) and its target gene, pepsinogen C (PGC), in gastric cancer (GC) and precancerous disease patients to evaluate their diagnostic efficiency for GC and its precursor and to investigate any correlation between the two. Serum samples were taken from 638 patients, including 214 GC patients, 222 atrophic gastritis (AG) patients, and 202 controls (CON). The expression of serum let-7 miRNA was detected in control-AG (precancerous disease) through to GC patients using quantitative reverse-transcription polymerase chain reaction. Serum PGC was determined by enzyme-linked immuno-sorbent assay. PGC expression in situ was detected by immunohistochemistry staining. The luciferase reporter gene system was used to verify correlation between let-7 miRNA and its predicted target gene. The results showed that serum let-7c, let-7i, and let-7f demonstrated significant differences in the CON-AG-GC sequence (P = 0.017, P < 0.001, P = 0.003, respectively); let-7c was significantly lower in the AG group, and let-7i and let-7f were significantly higher in the GC group. Significantly different expressions of serum PGC were found among the three diseases, and also between AG vs. CON, and GC vs. CON (P = 0.027, P = 0.001, respectively). Linear-regression analysis suggested that serum let-7c was negatively correlated to the expression of PGC (r = -0.096, P = 0.047), and serum let-7c, let-7i, and let-7f showed no association with PGC expression in tissue. In addition, serum let-7c, let-7f, and let-7i showed significant correlations with environment factors. Serum let-7c, let-7i, and let-7f demonstrated significant differences in the CON-AG-GC disease sequence indicating that let-7 miRNA might have value by serving as potential biomarker in the diagnosis of GC or its precancerous diseases. There were significant negative correlations between serum let-7c and its target gene PGC expression.
机译：这项研究检查了血清let-7 microRNA（miRNA）及其靶基因胃蛋白酶原C（PGC）在胃癌（GC）和癌前疾病患者中的表达模式，以评估其对GC及其前体的诊断效率，并调查两者之间的相关性。血清样本来自638例患者，包括214例GC患者，222例萎缩性胃炎（AG）和202例对照（CON）。使用定量逆转录聚合酶链反应，在对照AG（癌前疾病）到GC患者中检测到血清let-7 miRNA的表达。通过酶联免疫吸附测定法测定血清PGC。通过免疫组织化学染色检测到PGC的原位表达。荧光素酶报告基因系统用于验证let-7 miRNA及其预测的靶基因之间的相关性。结果表明，血清let-7c，let-7i和let-7f在CON-AG-GC序列中显示出显着差异（分别为P = 0.017，P <0.001，P = 0.003）; AG组中let-7c显着降低，GC组中let-7i和let-7f显着升高。在这三种疾病之间，以及AG vs. CON和GC vs. CON之间，血清PGC的表达差异显着（分别为P = 0.027，P = 0.001）。线性回归分析表明，血清let-7c与PGC的表达呈负相关（r = -0.096，P = 0.047），血清let-7c，let-7i和let-7f与PGC的表达无关。组织。此外，血清let-7c，let-7f和let-7i与环境因素之间存在显着相关性。血清let-7c，let-7i和let-7f在CON-AG-GC疾病序列中显示出显着差异，表明let-7 miRNA通过作为潜在的生物标志物诊断GC或其癌前疾病可能具有价值。血清let-7c与其靶基因PGC表达之间存在显着的负相关。

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