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Phage display in the study of infectious diseases

机译:噬菌体展示在传染病研究中的应用

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Microbial infections are dependent on the panoply of interactions between pathogen and host and identifying the molecular basis of such interactions is necessary to understand and control infection. Phage display is a simple functional genomic methodology for screening and identifying protein-ligand interactions and is widely used in epitope mapping, antibody engineering and screening for receptor agonists or antagonists. Phage display is also used widely in various forms, including the use of fragment libraries of whole microbial genomes, to identify peptide-ligand and protein-ligand interactions that are of importance in infection. In particular, this technique has proved successful in identifying microbial adhesins that are vital for colonization.
机译:微生物感染取决于病原体与宿主之间相互作用的全过程,因此识别这种相互作用的分子基础对于理解和控制感染是必要的。噬菌体展示是一种用于筛选和鉴定蛋白质-配体相互作用的简单功能基因组学方法,广泛用于表位作图,抗体工程和受体激动剂或拮抗剂的筛选。噬菌体展示还以各种形式广泛使用,包括使用整个微生物基因组的片段库来鉴定在感染中很重要的肽-配体和蛋白质-配体相互作用。特别地,已证明该技术成功地鉴定了对定殖至关重要的微生物粘附素。

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