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首页> 外文期刊>Trends in Microbiology >The rpoB gene as a tool for clinical microbiologists
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The rpoB gene as a tool for clinical microbiologists

机译：rpoB基因作为临床微生物学家的工具

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摘要

The rpoB gene, encoding the beta -subunit of RNA polymerase, has emerged as a core gene candidate for phylogenetic analyses and identification of bacteria, especially when studying closely related isolates. Together with the 16S rRNA gene, rpoB has helped to delineate new bacterial species and refine bacterial community analysis, as well as enabling the monitoring of rifampicin resistance-conferring mutations. Sequencing of rpoB enables efficient estimation of bacterial G+C% content, DNA-DNA hybridization value and average nucleotide identity (percentage of the total genomic sequence shared between two strains) when taxonomic relationships have been firmly established. New identification tools targeting a rpoB gene fragment located between positions 2300 and 3300 have been developed recently. Therefore, inclusion of the rpoB gene sequence would be useful when describing new bacterial species.
机译：编码RNA聚合酶β亚基的rpoB基因已成为细菌系统发育分析和鉴定的核心基因候选者，特别是在研究密切相关的分离株时。 rpoB与16S rRNA基因一起，帮助描绘了新的细菌种类并完善了细菌群落分析，并能够监测赋予利福平耐药性的突变。牢固建立分类学关系后，对rpoB进行测序可有效评估细菌G + C％含量，DNA-DNA杂交值和平均核苷酸同一性（两个菌株之间共享的总基因组序列的百分比）。最近已经开发出了针对位于位置2300和3300之间的rpoB基因片段的新识别工具。因此，当描述新的细菌种类时，包含rpoB基因序列将是有用的。

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