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When metagenomics meets stable-isotope probing: progress and perspectives

机译:当宏基因组学与稳定同位素探测相遇时:进展与展望

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The application of metagenomics, the culture-independent capture and subsequent analysis of genomic DNA from the environment, has greatly expanded our knowledge of the diversity of microbes and microbial protein families; however, the metabolic functions of many microorganisms remain largely unknown. DNA stable-isotope probing (DNA-SIP) is a recently developed method in which the incorporation of stable isotope from a labelled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds.
机译:宏基因组学的应用,与培养无关的捕获以及对环境中基因组DNA的后续分析,极大地扩展了我们对微生物和微生物蛋白家族多样性的认识;然而,许多微生物的代谢功能仍然是未知的。 DNA稳定同位素探测(DNA-SIP)是最近开发的一种方法,其中从标记的底物中掺入稳定同位素用于鉴定环境中微生物的功能。该技术现已与宏基因组学结合使用,以在微生物特性和特定代谢功能之间建立联系。 DNA-SIP和宏基因组学的结合不仅允许从宏基因组库中检测稀有的低丰度物种,而且还有助于检测新型酶和生物活性化合物。

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