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首页> 外文期刊>Trends in Microbiology >Innovative techniques, sensors, and approaches for imaging biofilms at different scales
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Innovative techniques, sensors, and approaches for imaging biofilms at different scales

机译：用于对生物膜进行不同规模成像的创新技术，传感器和方法

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Confocal laser scanning microscopy has become a standard technique for the investigation of hydrated interfacial microbial communities at the microscale. Multiphoton and spinning-disk microscopes provide new options for in situ imaging. Progress has been made in imaging structural aspects as well as interactions and processes. Advanced fluorescence techniques such as lifetime imaging and correlation spectroscopy are also available. Newly developed target-specific probes allow investigation of new aspects of microbial communities. Several new laser-based techniques are available including nanoscopy and mesoscale techniques. Nanoscopy techniques offer access to unprecedented resolution of hydrated microbiological samples at the scale of fluorescent gene products and macromolecules. Mesoscale approaches are important to address larger features and statistical issues of microbiological samples. This review presents the state of the art in situ biofilm imaging and assesses the pros and cons of laser-based imaging techniques in combination with a variety of sensor types at different scales.
机译：共聚焦激光扫描显微镜已成为在微观尺度上研究水合界面微生物群落的标准技术。多光子显微镜和旋转盘显微镜为原位成像提供了新的选择。在成像结构方面以及相互作用和过程方面已取得进展。也可以使用先进的荧光技术，例如寿命成像和相关光谱。最新开发的目标特异性探针可以研究微生物群落的新方面。有几种基于激光的新技术可供使用，包括纳米技术和中尺度技术。纳米技术可以以荧光基因产物和大分子的规模获得水合微生物样品前所未有的分辨率。中尺度方法对于解决微生物样品的较大特征和统计问题很重要。这篇综述介绍了原位生物膜成像技术的现状，并评估了基于激光的成像技术与各种规模的各种传感器相结合的利弊。

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