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首页> 外文期刊>Tropical Animal Health and Production >Detection of virulence-associated genes in Staphylococcus aureus isolated from bovine clinical mastitis milk samples in Guangxi
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Detection of virulence-associated genes in Staphylococcus aureus isolated from bovine clinical mastitis milk samples in Guangxi

机译：广西牛临床乳腺炎牛奶样品中分离的金黄色葡萄球菌中毒力相关基因的检测

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摘要

Staphylococcus aureus is recognized worldwide as a pathogen causing many serious diseases in humans and animals and is one of the most common etiological agents of clinical and subclinical bovine mastitis. The purpose of this study was to determine the presence of genes encoding clfA, fnbA, fnbB, cap5, cap8, hla, hlb, nuc, sea, and tst of S. aureus strains (n=39) isolated from bovine clinical mastitis in Guangxi by polymerase chain reaction amplification. The results of the present study indicated that all isolates were found to contain one or more virulence-associated genes. The most frequently encountered genes were fnbA (97%) and nuc (90%), followed by hla (85%) and hlb (82%), respectively. None of the investigated S. aureus strains harbored fnbB and sea genes. The data in the present study showed a relatively wide distribution of the genes fnbA and nuc among the investigated isolates, indicating that they play an important role on bovine mastitis pathogenesis. The study provides a valuable insight into the virulence-associated genes of this important pathogen.Digital Object Identifier http://dx.doi.org/10.1007/s11250-012-0143-z
机译：金黄色葡萄球菌被公认为引起人类和动物许多严重疾病的病原体，并且是临床和亚临床牛乳腺炎最常见的病原体之一。这项研究的目的是确定从广西牛临床乳腺炎分离的金黄色葡萄球菌菌株（n = 39）的编码clfA，fnbA，fnbB，cap5，cap8，hla，hlb，nuc，sea和tst的基因的存在通过聚合酶链反应扩增。本研究的结果表明，所有分离株都含有一个或多个毒力相关基因。最常见的基因是fnbA（97％）和nuc（90％），其次是hla（85％）和hlb（82％）。被调查的金黄色葡萄球菌菌株均未携带fnbB和海洋基因。本研究中的数据显示，fnbA和nuc基因在所研究的分离株中分布相对较广，表明它们在牛乳腺炎的发病机理中起着重要作用。该研究为这种重要病原体的毒力相关基因提供了宝贵的见解。数字对象标识符http://dx.doi.org/10.1007/s11250-012-0143-z

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