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首页> 外文期刊>Tropical Animal Health and Production >Prevalence, molecular diagnosis and treatment of Mycoplasma conjunctivae isolated from infectious keratoconjunctivitis affected Lohi sheep maintained at Livestock Experiment Station, Bahadurnagar, Okara, Pakistan
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Prevalence, molecular diagnosis and treatment of Mycoplasma conjunctivae isolated from infectious keratoconjunctivitis affected Lohi sheep maintained at Livestock Experiment Station, Bahadurnagar, Okara, Pakistan

机译：从感染角膜结膜炎的Lohi绵羊分离出的结膜支原体的流行，分子诊断和治疗，保存在巴基斯坦奥卡拉巴哈杜那那家畜实验站

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摘要

Mycoplasma conjunctivae are etiological agents of infectious keratoconjunctivitis (IKC), commonly known as pink-eye in domestic sheep, goats and other wild animals in many parts of the world. A few young Lohi lambs maintained at Livestock Experiment Station (LES), Bahadurnagar, Okara, Pakistan showed clinical signs and symptoms of conjunctivitis, keratitis, severe lacrimation and varying degree of blindness. During January to March, 2011, a total of 36 ocular swabs were collected from IKC affected animals and were processed for isolation, identification, and characterization of M. conjunctivae. Sixteen (44.44 %) out of 36 samples showed turbidity in PPLO broth. Twelve (75 %) out of 16 broth samples showed colony growth on PPLO agar. All 16 (44.44 %) out of 36 turbid broth samples, 12 (75 %) out of 16 cultured on agar plate samples, and 21 (59 %) out of 36 sheep ocular direct swab samples were found positive for M. conjunctivae through polymerase chain reaction test by using M. conjunctivae-specific primer pair McoF1 and McoR1 and detecting a 750 base pair fragment on agarose gel. Topical application of 0.5 % sterile solution of gentamycin (100 mg/ml) (Gentafar 10 %, FARVET, Netherlands) proved suitable for the treatment of IKC in Lohi lambs as all clinical signs of IKC disappeared after 5 days of treatment with this antibiotic. This is the first report about the prevalence, molecular diagnosis, and treatment of M. conjunctivae in Lohi sheep affected with infectious keratoconjunctivitis at LES, Bahadurnagar, Okara, Pakistan.
机译：结膜支原体是感染性角结膜炎（IKC）的病原体，在世界许多地方的家养绵羊，山羊和其他野生动物中通常被称为红眼病。巴基斯坦奥卡拉巴哈杜那那加牲畜实验站（LES）饲养的几只幼年Lohi羔羊表现出结膜炎，角膜炎，严重流泪和不同程度的失明的临床体征和症状。在2011年1月至3月期间，共从IKC感染的动物中收集了36个眼拭子，并对其进行处理以进行结膜分枝杆菌的分离，鉴定和鉴定。 36个样本中有16个（44.44％）在PPLO肉汤中显示出浑浊。 16个肉汤样品中有12个（75％）在PPLO琼脂上显示菌落生长。在36个混浊肉汤样品中，所有16个（44.44％），在琼脂平板样品上培养的16个中，有12个（75％），在36个绵羊眼直接拭子样品中，有21个（59％）通过聚合酶对结膜分支杆菌呈阳性通过使用结膜分枝杆菌特异性引物对McoF1和McoR1并在琼脂糖凝胶上检测到750个碱基对片段进行链反应测试。局部应用0.5％庆大霉素无菌溶液（100 mg / ml）（Gentafar 10％，FARVET，荷兰）证明适用于Lohi羔羊IKC的治疗，因为用这种抗生素治疗5天后，IKC的所有临床症状均消失了。这是巴基斯坦巴哈杜那那尔市LES感染Lohi绵羊的结膜分支杆菌患病率，分子诊断和治疗的首次报道。

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