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首页> 外文期刊>Tropical Animal Health and Production >Molecular detection of Cryptosporidium spp. infections in water buffaloes from northeast Thailand
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Molecular detection of Cryptosporidium spp. infections in water buffaloes from northeast Thailand

机译：隐孢子虫的分子检测。泰国东北部水牛的感染

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摘要

The objectives of this study were to determine the individual and herd-level prevalence and genotype of Cryptosporidium and to identify putative risk factors associated with Cryptosporidium spp. infections in water buffaloes in northeast Thailand. Fecal samples from 600 water buffaloes of 287 farms in six provinces were collected and tested using DMSO-modified acid-fast staining and polymerase chain reaction. The overall prevalence of Cryptosporidium infections in buffaloes was 5.7 and 8.7 % among individual animals and herds, respectively. The provinces with highest infected Cryptosporidium were located in the Sakon Nakhon Basin in the northern part of the region. In addition, higher herd prevalence was observed among farms with more than five buffaloes (30 %) than those with five or less animals (16.2 %). Thirty (88.2 %) of the 34 Cryptosporidium-positive samples were Cryptosporidium parvum and four (11.8 %) were Cryptosporidium ryanae.
机译：这项研究的目的是确定隐孢子虫的个体和畜群水平的流行率和基因型，并确定与隐孢子虫相关的推定危险因素。泰国东北部水牛的感染。收集了来自六个省的287个农场的600只水牛的粪便样本，并使用DMSO修饰的耐酸染色和聚合酶链反应进行了测试。水牛中隐孢子虫感染的总体患病率在个别动物和畜群中分别为5.7％和8.7％。隐孢子虫感染率最高的省份位于该地区北部的沙空那空盆地。此外，在水牛数量超过五个的农场（30％）中，牛群的患病率高于五个或以下动物的农场（16.2％）。 34个隐孢子虫阳性样本中有30个（88.2％）是小隐孢子虫，而四个（11.8％）是黑隐隐孢子虫。

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