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Evaluation of hepatitis E virus infection between different production systems of pigs in Brazil

机译：巴西不同生产系统之间戊型肝炎病毒感染的评估

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The objectives of this study were to (1) investigate the occurrence of hepatitis E virus (HEV) in pigs from large-scale and family-scale farms, (2) genetically characterize the strains isolated, and (3) study the pathogenesis of swine HEV infection via immunohistochemistry. A total of 50 pigs from 10 farms in Mato Grosso State, Brazil were divided according to type of production system into either large-scale farms (n = 5) or family-scale farms (n = 5). Samples of liver, gallbladder, small and large intestines, bile, and feces from the pigs were analyzed by nested PCR with primers targeting the ORF2 region of HEV and by immunohistochemistry. Of the eight HEV-positive samples from pigs of family-scale farms, phylogenetic analysis revealed that seven of the swine HEV isolates clustered with subtype 3b of genotype 3 and one isolate was categorized with subtype 3 f. The HEV antigen was detected mainly in the small intestine samples from family-scale farms, suggesting an early stage HEV infection. HEV was not detected in the samples of pigs from large-scale farms, reinforcing the need for additional studies to evaluate the risk of transmission of HEV to humans from pigs from family-scale farms in Mato Grosso State.
机译：这项研究的目的是（1）研究大型和家庭规模猪的戊型肝炎病毒（HEV）的发生;（2）对分离出的毒株进行遗传鉴定;以及（3）研究猪的发病机理通过免疫组织化学进行HEV感染。根据生产系统的类型，来自巴西马托格罗索州10个农场的总共50头猪被分为大型农场（n = 5）或家庭规模农场（n = 5）。猪的肝脏，胆囊，小肠和大肠，胆汁和粪便样品通过巢式PCR（针对HEV ORF2区的引物）和免疫组织化学进行分析。在家庭规模农场的猪的八种戊型肝炎病毒阳性样本中，系统发育分析表明，猪戊型肝炎病毒分离株中有七种与基因型3b亚型3b聚在一起，一种分离株被归为3f亚型。 HEV抗原主要在家庭规模农场的小肠样本中检测到，表明是早期HEV感染。在大型农场的猪样本中未检测到戊型肝炎病毒，这增加了对进一步研究的需要，以评估马托格罗索州家庭规模农场的猪向人类传播戊型肝炎病毒的风险。

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