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首页> 外文期刊>Tropical Animal Health and Production >Vaccination of chickens using raw rice coated with novel trehalose nano-organogels containing Newcastle disease (strain I-2) vaccine
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Vaccination of chickens using raw rice coated with novel trehalose nano-organogels containing Newcastle disease (strain I-2) vaccine

机译：使用涂有新型海藻糖纳米有机凝胶（包含新城疫（I-2株）疫苗）的生米接种鸡的疫苗

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The formulation and evaluation of trehalose nano-organogels for storage and oral delivery of Newcastle disease (ND) strain I-2 vaccine to chickens were carried out in this study. Trehalose sugar was blended with vegetable oil to form nano-organogels where trehalose also acted as a stabilizer against thermal inactivation of I-2 ND virus. Results from infectivity titration assay indicated that the titre of 10(7.5) EID(50)/0.1 mL was maintained after 12 weeks of storage of nano-organogel I-2 vaccine at ambient room temperature. Serology results showed that 33% chickens which were vaccinated with nano-organogel I-2 vaccine after 14 days had HI antibody titres of >/= 3.0 log(2) with GMT of 2.3. Moreover, results showed 100% of chickens vaccinated with nano-organogel I-2 vaccine had the mean antibody titres of 3.4 and 3.7 log(2) at 21 and 28 days after vaccination, respectively. All vaccinated chickens (100%) survived the challenge of virulent ND virus whereas all unvaccinated chickens succumbed to challenge and died of signs consistent with ND. The findings from this study showed that the nano-organogel I-2 vaccine was stable at room temperature, safe and produced protective antibody response in vaccinated chickens. Moreover the nano-organogel I-2 vaccine was used for oral administration and hence is suitable for mass vaccination. However, optimization of the formulation of trehalose nano-organogel vaccine is required in order to achieve its application potentials.
机译：在本研究中，进行了海藻糖纳米有机凝胶的配制和评估，用于储存和口服鸡新城疫（ND）I-2疫苗。海藻糖与植物油混合形成纳米有机凝胶，其中海藻糖还可以作为抗I-2 ND病毒热失活的稳定剂。感染性滴定分析的结果表明，在室温下储存12周的纳米有机凝胶I-2疫苗后，滴度保持在10（7.5）EID（50）/0.1 mL。血清学结果显示，在14天后接种纳米有机凝胶I-2疫苗的33％的鸡的HI抗体滴度> / = 3.0 log（2），GMT为2.3。此外，结果显示，接种纳米有机凝胶I-2疫苗的鸡100％在接种后21天和28天分别具有3.4和3.7 log（2）的平均抗体滴度。所有接种疫苗的鸡（100％）在有毒力的ND病毒的攻击下均存活，而所有未接种疫苗的鸡都屈服于攻击并死于与ND一致的体征。这项研究的结果表明，纳米有机凝胶I-2疫苗在室温下稳定，安全，并在接种鸡中产生保护性抗体反应。此外，纳米有机凝胶I-2疫苗用于口服给药，因此适合大规模疫苗接种。然而，为了实现其应用潜力，需要优化海藻糖纳米有机凝胶疫苗的配方。

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