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首页> 外文期刊>Tropical Animal Health and Production >Molecular identification of chlamydial cause of abortion in small ruminants in Jordan
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Molecular identification of chlamydial cause of abortion in small ruminants in Jordan

机译：约旦小反刍动物衣原体流产原因的分子鉴定

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摘要

Chlamydophila abortus (Ch. abortus) is the etiological agent of ovine enzootic abortion (OEA) and one of the most common infectious agents of abortion in small ruminants worldwide. RFLP-PCR analysis of the outer membrane protein gene (OMP2 gene) was used for diagnosis and characterization of chlamydial causes of abortion in small ruminants in Jordan. Sixty-six placental tissues and 15 vaginal swabs were collected from aborted ewes and does to identify cause of abortion in Jordan. Thirty-eight placental samples (58 %) and 13 vaginal swabs (87 %) were positive for chlamydial DNA. Shedding of bacteria in vaginal swabs was detected within 7 days after abortion. The results of this study showed that chlamydiosis is one of the important causes of abortion in small ruminants in Jordan. In addition, vaginal swab is an excellent sample for molecular diagnosis of chlamydiosis. DNA sequencing and RFLP analysis of the OMP2 reveal that all chlamydial cause of abortion in small ruminants in Jordan are due to Ch. abortus. While, Ch. pecorum was not detected in any sample. OMP2 gene of the isolated Jordanian strain was identical (100 %) to Ch. abortus FAS strain. In conclusion, Ch. abortus is an important cause of abortion in Jordan; vaginal swab within 7 days of abortion can be used for molecular diagnosis of chlamydiosis in small ruminants.
机译：流产衣原体（Ch。Chrydophila）是绵羊动物性流产（OEA）的病原体，是全世界小型反刍动物中最常见的流产传染原之一。外膜蛋白基因（OMP2基因）的RFLP-PCR分析被用于约旦小反刍动物衣原体流产原因的诊断和鉴定。从流产的母羊中收集了66个胎盘组织和15个阴道拭子，并确定了约旦的流产原因。 38个胎盘样本（58％）和13个阴道拭子（87％）呈衣原体DNA阳性。流产后7天内检测到阴道拭子中有细菌脱落。这项研究的结果表明，衣原体病是约旦小反刍动物流产的重要原因之一。此外，阴道拭子是用于衣原体病分子诊断的极佳样品。 DNA测序和OMP2的RFLP分析表明，约旦小反刍动物中所有衣原体流产的原因都是Ch。流产。一会儿在任何样品中均未检测到pecorum。分离的约旦菌株的OMP2基因与Ch。（100％）相同。流产FAS株。最后，Ch。流产是约旦流产的重要原因。流产后7天内的阴道拭子可用于小反刍动物披衣菌病的分子诊断。

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