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首页> 外文期刊>Tropical Animal Health and Production >Molecular characterization of Newcastle disease viruses isolated from rural chicken in northwest Ethiopia reveals the circulation of three distinct genotypes in the country
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Molecular characterization of Newcastle disease viruses isolated from rural chicken in northwest Ethiopia reveals the circulation of three distinct genotypes in the country

机译：从埃塞俄比亚西北部农村鸡中分离出的新城疫病毒的分子特征揭示了该国三种不同基因型的流行

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摘要

Newcastle disease (ND) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide. Fifteen Newcastle disease virus (NDV) isolates obtained from rural chickens in northwest Ethiopia in 2011 and 2012 were characterized genotypically. The main functional region of the F gene was amplified and sequenced (260 nucleotides). Among the Ethiopian NDV isolates, 2 isolates had the virulent motif R-112-R-Q-K-R-F-117 at the cleavage site of the fusion protein while 13 isolates contained the lentogenic motif (112)G-G/R-Q-G-R-L-117. Phylogenetic analysis based on the variable region of the F gene indicated that the two isolates exhibiting the virulent motif belonged to lineage 5 (genotype VII) subgenotype d and the remaining 13 isolates were grouped into lineage 2 (genotype II). The nucleotide sequences of lineage 5 isolates were genetically related to the Sudanese NDV isolates, suggesting potential epidemiological link of ND outbreaks between neighbouring countries. The lentogenic strains shared similarities with La Sota vaccine strain and probably originated from the vaccine strain either through direct exposure of birds to the live vaccine or to infectious La Sota-like strains circulating in rural poultry. This study provides genetic evidence on the existence of different NDV genotypes circulating in the rural poultry in Ethiopia. The virulent NDV continues to be a problem in poultry sector in Ethiopia, and their continuous circulation in rural and commercial poultry calls for improved surveillance and intensified vaccination and other control measures.
机译：新城疫（ND）是一种高度传染性疾病，会影响许多鸟类，并给全世界的家禽业造成重大经济损失。从基因型上鉴定了2011年和2012年从埃塞俄比亚西北部农村鸡中获得的15株新城疫病毒（NDV）分离株。 F基因的主要功能区被扩增和测序（260个核苷酸）。在埃塞俄比亚NDV分离株中，有2个分离株在融合蛋白的裂解位点具有强毒基序R-112-R-Q-K-R-F-117，而13个分离株则含有变长型（112）G-G / R-Q-G-R-L-117。基于F基因的可变区的系统发育分析表明，显示有毒力的两个分离株属于谱系5（基因型VII）的亚基因d，其余13个分离株被分为谱系2（基因型II）。谱系5分离株的核苷酸序列与苏丹NDV分离株遗传相关，表明邻国之间ND暴发的潜在流行病学联系。慢病毒菌株与La Sota疫苗菌株具有相似之处，并且可能源于该疫苗菌株，要么是禽类直接暴露于活疫苗，要么是农村家禽中传播的传染性La Sota样菌株。这项研究提供了埃塞俄比亚农村家禽中存在的不同NDV基因型的遗传证据。在埃塞俄比亚，强毒新城疫仍然是家禽业的一个问题，它们在农村和商业家禽中的不断传播要求改善监测，加强疫苗接种和其他控制措施。

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