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Pathogenicity evaluation of different Newcastle disease virus chimeras in 4-week-old chickens

机译：不同新城疫病毒嵌合体在4周龄鸡中的致病性评估

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摘要

The aim of this study was to evaluate the disease-inducing ability of four chimeric Newcastle disease viruses (NDV) by clinicopathological assessment. The infectious clones were previously generated by insertion of hemagglutinin-neuraminidase (HN) and/or fusion (F) genes from virulent strains (Turkey North Dakota and California 02) into a mesogenic strain (Anhinga) backbone. Groups of 4-week-old chickens were inoculated via eye drop instillation, clinical signs were monitored daily, and necropsies with collection of tissues were performed at 2, 5, 10, and 14 days post infection. Tissue sections were evaluated for histopathology and immunohistochemistry for NDV nucleoprotein. All viruses replicated successfully in the natural host, although viral recovery, seroconversion, and extent of immunohistochemical staining were greatest from birds infected with those viruses containing both F and HN genes from the same virulent virus. There was minimal to no increase in clinicopathologic disease due to infection with the chimeras compared to the recombinant backbone. However, all birds developed histological evidence of encephalitis. The results suggest that the inherent virulence of Turkey North Dakota and California 2002 strains is due to more than the simple presence of their F and HN genes.
机译：这项研究的目的是通过临床病理评估来评估四种嵌合新城疫病毒（NDV）的疾病诱导能力。感染性克隆以前是通过将血凝素神经氨酸酶（HN）和/或融合（F）基因从强毒菌株（土耳其北达科他州和加利福尼亚02）插入介晶菌株（Anhinga）的骨架中而产生的。通过滴眼液接种成组的4周龄鸡，每天监测临床体征，并在感染后第2、5、10和14天进行尸检并收集组织。评价组织切片的NDV核蛋白的组织病理学和免疫组化。所有病毒均能在天然宿主中成功复制，尽管病毒感染，血清转换和免疫组织化学染色的程度最大，而这些鸟同时感染了来自同一毒性病毒的F和HN基因。与重组骨架相比，由于嵌合体感染引起的临床病理学疾病的增加极少或没有。但是，所有鸟类都发展出脑炎的组织学证据。结果表明，土耳其北达科他州和加利福尼亚州2002菌株的固有毒力不仅仅是其F和HN基因的简单存在。

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