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首页> 外文期刊>Tropical Animal Health and Production >Seroprevalence and molecular detection of porcine sapovirus in symptomatic suckling piglets in Guangdong Province, China
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Seroprevalence and molecular detection of porcine sapovirus in symptomatic suckling piglets in Guangdong Province, China

机译：广东省有症状哺乳仔猪猪瘟病毒的血清阳性率和分子检测

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The seroprevalence and genetic identification of sapovirus (SaV) in symptomatic suckling piglets were investigated in Guangdong Province, China, between November 2011 and April 2013. Serum (n=960) and diarrheic fecal (n=101) samples collected from symptomatic suckling piglets in Guangdong Province were evaluated for antibodies against SaV using indirect enzyme-linked immunosorbent assay (iELISA). The overall seroprevalence of SaV in symptomatic suckling piglets was 61.9 % (594/960). Positive animals were found in all regions with seroprevalence ranging from 52 to 67.8 %, but the difference was not statistically significant (P>0.05). In addition, RNA of SaV was extracted from diarrheic fecal samples, and the partial polymerase gene was amplified by RT-PCR and then sequenced. Seven of 101 (6.9 %) samples were found to contain porcine SaV. Phylogenetic analysis showed that all the porcine SaV isolates belong to the porcine SaV genogroup III (GIII). This is the first report of SaV seroprevalence in symptomatic pigs in China.
机译：2011年11月至2013年4月，在中国广东省对有症状的乳猪进行了血清病毒的Sav流行和遗传鉴定。从有症状的乳猪的血清（n = 960）和腹泻性粪便（n = 101）中采集了血清。使用间接酶联免疫吸附试验（iELISA）对广东省的SaV抗体进行了评估。有症状的乳猪的SaV总体血清阳性率为61.9％（594/960）。在所有地区均发现阳性动物，血清阳性率在52％至67.8％之间，但差异无统计学意义（P> 0.05）。另外，从腹泻的粪便样品中提取SaV的RNA，并通过RT-PCR扩增部分聚合酶基因，然后进行测序。发现101个样本中有七个（6.9％）包含猪SaV。系统发育分析表明，所有猪SaV分离株均属于猪SaV基因组III（GIII）。这是中国有症状猪中SaV血清阳性率的首次报道。

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