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首页> 外文期刊>Tropical Animal Health and Production >The detection of Candidatus Mycoplasma haemobos in cattle and buffalo in China
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The detection of Candidatus Mycoplasma haemobos in cattle and buffalo in China

机译：中国牛，水牛支原体血友病杆菌的检测

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Candidatus Mycoplasma haemobos is a hemoplasma species found in cattle and has been recently reported in Switzerland and Japan. In this study, Candidatus Mycoplasma haemobos was shown to occur in cattle and buffalo in tropical China by PCR amplification and sequence analysis of the 16S rRNA gene from blood samples. Based on the 16S rDNA sequence, a specific PCR assay was developed. Occurrence of Candidatus Mycoplasma haemobos in cattle and buffalo in Guangxi, China, was determined by examining 25 buffalo blood samples, 12 yellow cattle blood samples and 42 dairy cow blood samples. The results showed that 32% (8/25) of buffalo, 41.7% (5/12) of yellow cattle, and 14.3% (6/42) of dairy cows were positive for Candidatus Mycoplasma haemobos, respectively. Direct sequencing of representative PCR products confirmed that the amplified partial 16S rDNA sequence represented Candidatus Mycoplasma haemobos. This is the first report of Candidatus Mycoplasma haemobos in buffalo, yellow cattle, and dairy cows in China.
机译：念珠菌支原体是牛中的血统物种，最近在瑞士和日本有报道。在这项研究中，通过PCR扩增和血样中16S rRNA基因的序列分析，证明了中国热带地区的牛和水牛中存在支原体支原体。基于16S rDNA序列，开发了一种特异性PCR检测方法。通过检查25头水牛血样，12头黄牛血样和42头奶牛血样，确定了中国广西牛和水牛中支原体支原体的发生率。结果显示，水牛念珠菌支原体呈阳性，水牛为32％（8/25），黄牛为41.7％（5/12），乳牛为14.3％（6/42）。代表性PCR产物的直接测序证实，扩增的部分16S rDNA序列代表了支原体支原体。这是中国水牛，黄牛和奶牛中支原体血友病杆菌的首次报道。

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