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The occurrence of the feline Candidatus Mycoplasma haemominutum in dog in China confirmed by sequence-based analysis of ribosomal DNA

机译:基于序列分析的核糖体DNA证实中国犬类血友病支原体的发生

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摘要

In the present study, polymerase chain reaction (PCR) assay was used to detect haemoplasmas (haemotropic bacteria) in 40 clinically healthy pet dogs in Foshan city, Guangdong Province, China, and one dog was found positive. Comparison of its 16S ribosomal DNA (rDNA) sequence with relevant sequences showed that the isolated haemoplasma had greater sequence identity to feline species Candidatus Mycoplasma haemominutum (99%) than to Candidatus Mycoplasma haematoparvum (95%). This result, for the first time, indicates the presence of the feline Candidatus Mycoplasma haemominutum in Chinese dogs and it represents the first survey of its kind in China by using PCR assay. The results indicated that dog may represent one of the hosts for the feline Candidatus Mycoplasma haemominutum.
机译:在本研究中,采用聚合酶链反应(PCR)分析法检测了广东省佛山市40例临床健康的宠物犬的血友病(嗜血细菌),并发现其中1只为阳性。将其16S核糖体DNA(rDNA)序列与相关序列进行比较后发现,分离出的血质与猫科念珠菌支原体(99%)的同源性高于对血友病支原体(95%)的支配。该结果首次表明中国犬中存在猫科支原体血友病,并且这是中国首次使用PCR法进行的同类调查。结果表明,狗可能是猫支原体支原体的宿主之一。

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