• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >Detection of sarcocystosis in goats in Malaysia by light microscopy, histology, and PCR
【24h】

Detection of sarcocystosis in goats in Malaysia by light microscopy, histology, and PCR

机译：通过光学显微镜，组织学和PCR检测马来西亚山羊的结节病

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A number of methods have been used for the detection of the presence of microsarcocysts in animals, but little information exists on the value between the various methods. This study therefore examined for Sarcocystis spp. using three different methods in 105 samples of skeletal muscle collected from goats slaughtered in an abattoir in Selangor, Malaysia from January to February 2014. Three methods were used, direct light microscopy of squashed fresh muscle tissues; histological examination of fixed, sectioned, and hematoxylin and eosin (H&E)-stained samples of muscle; and molecular identification by polymerase chain reaction (PCR). Of the 105 tissue samples, 55 (52.38 %) were positive by light microscopy (LM), 46 (43.8 %) by histology, and 95 (90.48 %) by PCR. Only 29 (27.6 %) and 5 (4.76 %) samples were positive and negative, respectively, by all three methods. The cysts were elongated to a spindle shape with a mean size of 393.30 x 81.6 mu m and containing banana-shaped bradyzoites of size 12.32 x 2.08 mu m. The wall of the cyst was radially striated with a thickness of 2.83 mu m. Samples were tested for the presence of Sarcocystis-specific 18S rRNA and were identified as Sarcocystis capracanis. Of the three methods used, the PCR test appears to be the most useful method for the diagnosis of sarcocystosis especially for species identification.
机译：已经使用了许多方法来检测动物中微囊藻的存在，但是各种方法之间的价值信息很少。因此，本研究检查了肉囊藻属。 2014年1月至2014年2月，从马来西亚雪兰莪屠宰场屠宰的山羊中采集的105块骨骼肌样本中，使用三种不同方法。固定，切片，苏木精和曙红（H＆E）染色的肌肉的组织学检查;通过聚合酶链反应（PCR）进行分子鉴定。在105个组织样本中，通过光学显微镜（LM）呈阳性的有55个（52.38％），通过组织学呈阳性的有46个（43.8％），通过PCR呈阳性的有95个（90.48％）。通过这三种方法，分别只有29个（27.6％）和5个（4.76％）样品为阳性和阴性。囊肿被拉长成纺锤形，平均大小为393.30 x 81.6微米，并包含大小为12.32 x 2.08微米的香蕉形缓殖子。囊肿壁呈放射状横纹，厚度为2.83微米。测试样品中是否存在Sarcocystis特异性18S rRNA，并将其鉴定为Sarcocystis capracanis。在使用的三种方法中，PCR检测似乎是诊断结节病的最有用方法，尤其是用于物种鉴定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号