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首页> 外文期刊>Tropical Animal Health and Production >Detection of Babesia bigemina infection in cattle from north-eastern India by polymerase chain reaction and its genetic relatedness with other isolates
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Detection of Babesia bigemina infection in cattle from north-eastern India by polymerase chain reaction and its genetic relatedness with other isolates

机译：聚合酶链反应检测印度东北部牛的重度巴贝虫感染及其与其他分离株的遗传关系

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A total of 333 blood samples were collected from cattle suspected for haemoprotozoan infections from three states of north-eastern part of India. All the samples were examined for diagnosis of Babesia bigemina infection using PCR for detection of specific DNA. Out of these, 12 (3.60 %) samples were found positive for B. bigemina DNA on PCR using the organism-specific primers derived from 18S ribosomal RNA (rRNA) gene of B. bigemina. An expected size of 1124-bp PCR product was visualized on agarose gel electrophoresis with all the 12 samples, and four of the products was further cloned and sequenced. Basic Local Alignment Search Tool (BLAST) analysis of B. bigemina sequences generated in the present study share 99.2 to 99.7 % identity at 18S rRNA gene nucleotide sequence level. These Indian B. bigemina sequences were found to be closely related with the cognate gene nucleotide sequences of B. bigemina from Argentina and Kenya where 99.1 to 99.9 % and 99.0 to 99.7 % nucleotide identities were observed, respectively. Distant relationship of these Indian organisms was observed with few cognate gene sequences from China where more than 7 % divergence was observed in the distance matrix.
机译：从印度东北部三个州的怀疑存在血原虫感染的牛中收集了总共333个血液样本。使用PCR检测特定DNA检查所有样品，以诊断大巴贝虫感染。在这些样本中，使用源自大双歧杆菌的18S核糖体RNA（rRNA）基因的生物特异性引物，在PCR上发现了12个（3.60％）的双歧杆菌DNA阳性。在所有12个样品上进行琼脂糖凝胶电泳时，可以看到预期大小为1124-bp的PCR产物，并且进一步克隆了四个产物并进行了测序。在本研究中生成的大双歧杆菌序列的基本局部比对搜索工具（BLAST）分析在18S rRNA基因核苷酸序列水平上具有99.2％至99.7％的同一性。发现这些印度双歧杆菌的序列与来自阿根廷和肯尼亚的双歧杆菌的同源基因核苷酸序列密切相关，分别观察到99.1％至99.9％和99.0％至99.7％的核苷酸同一性。观察到这些印度生物的远缘关系，而来自中国的同源基因序列很少，在距离矩阵中观察到的差异超过7％。

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