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The use of FAMACHA in estimation of gastrointestinal nematodes and total worm burden in Damara and Barbados Blackbelly cross sheep

机译:FAMACHA在估算达玛拉和巴巴多斯黑腹sheep羊的胃肠道线虫和蠕虫总负担中的应用

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The prevalence of gastrointestinal (GI) nematodes and total worm burden of Damara and Barbados Blackbelly cross sheep was investigated among smallholder farms in Salak Tinggi district of Selangor, Malaysia. A total of 50 sheep raised in smallholder farms comprising of 27 Damara cross and 23 Barbados Blackbelly cross were categorized based on their age into young and adults. Fecal samples were collected and examined for strongyle egg count by using modified McMaster technique. Severity of infection was categorized into mild, moderate, and heavy, based on egg per gram (EPG). Five sheep were randomly selected and slaughtered to examine the presence of adult gastrointestinal (GI) nematodes through total worm count (TWC). Faffa Malan Chart (FAMACHA) score was used for investigation of worm load based on the degree of anemia. The study revealed an overall EPG prevalence of 88 %, of which 84.1 % had mild infection. There was a significant difference (p = 0.002) in EPG among the two breeds. Based on age, significant difference (p = 0. 004) in EPG was observed among Barbados Blackbelly cross, but not for Damara cross (p = 0.941). The correlation between severity of infection and the FAMACHA score was significant (r = 0.289; p = 0.042). Haemonchus spp. were the most predominant nematode found in the gastrointestinal tract, followed by Trichostrongylus and Oesophagostomum spps. EPG and TWC for Haemonchus were positively correlated, but not significant (r = 0.85, p = 0.066). From regression analysis, 73 % of the variability in TWC for Haemonchus could be explained by EPG. Thus, it can be concluded that FAMACHA score correlates well with severity of infection of a nematode and can be used to assess the strongyle nematode burden in the different sheep crosses.
机译:在马来西亚雪兰莪州Salak Tinggi区的小农户中,对Damara和Barbados Blackbelly杂交羊的胃肠道(GI)线虫患病率和总蠕虫负担进行了调查。根据年龄的不同,在小农户农场中饲养的50只绵羊按年龄进行了分类,分为年幼和成年。收集粪便样本,并使用改良的McMaster技术检查其坚韧卵数。根据鸡蛋每克(EPG),感染的严重程度分为轻度,中度和重度。随机选择五只绵羊并宰杀以通过总蠕虫计数(TWC)检查成年胃肠道(GI)线虫的存在。 Faffa Malan Chart(FAMACHA)分数用于根据贫血程度调查蠕虫负荷。研究显示,总体EPG患病率为88%,其中84.1%为轻度感染。在两个品种中,EPG存在显着差异(p = 0.002)。根据年龄,在巴巴多斯·布莱克利(Barbelos Blackbelly)十字架之间观察到EPG的显着差异(p = 0. 004),但达玛拉十字架(Para)则没有观察到(p = 0.941)。感染严重程度与FAMACHA评分之间的相关性很显着(r = 0.289; p = 0.042)。葡萄球菌是在胃肠道中发现的最主要的线虫,其次是Trichostrongylus和Oesophagostomum spps。葡萄球菌的EPG和TWC呈正相关,但不显着(r = 0.85,p = 0.066)。通过回归分析,EPG可以解释Haemonchus在TWC中73%的变异性。因此,可以得出结论,FAMACHA评分与线虫感染的严重程度密切相关,可以用于评估不同绵羊杂交中的线虫线虫负担。

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