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The first report on the prevalence of pestivirus infection in camels in Sudan

机译:关于苏丹骆驼瘟病毒感染率的第一份报告

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The role of pestivirus particularly bovine viral diarrhea virus (BVDV) in causing respiratory infections in camels was studied in four different localities in Sudan. The evaluation was carried out using ELISA, and positive specimens were further tested using direct fluorescent antibody technique (FAT) and reverse transcriptase polymerase chain reaction (RT-PCR) for confirmation. The overall detected seroprevalence of BVD in camel sera was 84.6% with the highest prevalence in Western Sudan (92.5%) and with most of positives showing 2+ and 3+ titer. Out of 186 lung specimens examined for BVDV antigen, 13 were found positive (7%) with the highest prevalence in Central Sudan. All ELISA-positive specimens were positive using FAT and RT-PCR. To our knowledge, this is the first report for the detection of BVDV antigen and antibodies in camels in Sudan.
机译:在苏丹的四个不同地方,研究了瘟病毒,特别是牛病毒性腹泻病毒(BVDV)在引起骆驼呼吸道感染中的作用。使用ELISA进行评估,并使用直接荧光抗体技术(FAT)和逆转录酶聚合酶链反应(RT-PCR)对阳性样本进行进一步测试以进行确认。骆驼血清中检测到的BVD血清总流行率为84.6%,在苏丹西部的患病率最高(92.5%),大多数阳性显示2+和3+滴度。在检查的186份BVDV抗原肺标本中,有13份阳性(7%)在苏丹中部患病率最高。使用FAT和RT-PCR,所有ELISA阳性标本均为阳性。据我们所知,这是苏丹检测骆驼中BVDV抗原和抗体的第一份报告。

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