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首页> 外文期刊>Tropical Animal Health and Production >Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya
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Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya

机译：肯尼亚内罗毕达戈雷蒂小牛和牛中发现的隐孢子虫种类

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A total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl-Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twenty-five samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans.
机译：从肯尼亚内罗毕的城市环境中收集了来自296个奶牛饲养家庭的1,734头牛粪便样本。使用改良的Ziehl-Neelsen染色方法和免疫荧光测定法来鉴定那些隐孢子虫卵囊感染的样品。通过Sheather的蔗糖浮选法从粪便阳性样品中分离卵囊，并用盖玻片从浓缩物中提取卵囊。从124个对隐孢子虫呈阳性的粪便样品中提取基因组DNA，并将其用作巢式PCR 18S rRNA基因的模板。 25个样品（20％）隐孢子虫（Cryptosporidium）呈PCR阳性，成功克隆了24个PCR产物并测序。序列和系统发育分析确定了17个样品（68％）为小隐孢子虫样，四个样品（16％）为黑麦隐孢子虫，三个样品（12％）为安氏隐孢子虫，一个样品（4％）为人隐孢子虫。据我们所知，这是第一个报告来自肯尼亚的牛中的小隐孢子虫，安德森螺旋体和人参弯曲杆菌的基因分型研究。这项研究的结果表明，犊牛和牛的隐孢子虫感染可能是感染人类的​​潜在寄生虫人畜共患病病原体。

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