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Leptospirosis in pigs, dogs, rodents, humans, and water in an area of the Colombian tropics

机译:哥伦比亚热带地区猪,狗,啮齿动物,人和水中的钩端螺旋体病

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Leptospirosis is a reemerging zoonosis of global distribution and is one of the causes of hemorrhagic fevers in the tropics. We sought to determine seroprevalence in humans and animals and isolate Leptospira interrogans sensu lato in domestic animals, rodents, and water sources. The study was conducted in a tropical area of the middle SinA(0) in Cordoba, Colombia. In a prospective descriptive study, we collected blood and urine from pigs and dogs, sera from rural human workers, sera and kidney macerates of rodents, and water samples from environmental sources. We used microagglutination to screen for antibodies to 13 serovars. Strains were cultured on the Ellinghausen-McCullough-Johnson-Harris medium and confirmed by PCR amplifying lipL32 gene. Seroprevalence was 55.9 % in pigs, 35.2 % in dogs, and 75.8 % in humans; no antibody was detected, and no Leptospira were isolated from kidney macerates of rodents. Seven L. interrogans sensu lato strains were isolated: three from pigs, two from dogs, and two from water. High seroprevalence in pigs, dogs, and humans, concomitant to isolation of strains, demonstrates that in Cordoba, transmission exists among animals, the environment, and humans, which warrants the implementation of public health intervention measures to reduce the epidemiological impact of leptospirosis in the region.
机译:钩端螺旋体病是一种正在全球范围内重新出现的人畜共患病,是热带地区出血热的原因之一。我们试图确定人类和动物的血清阳性率,并从家畜,啮齿动物和水源中分离出问号钩端螺旋体。该研究是在哥伦比亚科尔多瓦中SinA(0)中部的热带地区进行的。在一项前瞻性描述性研究中,我们收集了猪和狗的血液和尿液,农村工人的血清,啮齿动物的血清和肾脏浸渍液以及来自环境的水样。我们使用微凝集来筛选针对13个血清型的抗体。菌株在Ellinghausen-McCullough-Johnson-Harris培养基上培养,并通过PCR扩增lipL32基因进行确认。猪的血清阳性率是55.9%,狗是35.2%,人是75.8%;没有检测到抗体,也没有从啮齿动物的肾脏浸出物中分离出钩端螺旋体。分离出七种问号L. interrogans sensu lato菌株:三株来自猪,两株来自狗,两株来自水。猪,狗和人的血清阳性率很高,伴随菌株的分离,表明在科尔多瓦,动物,环境和人类之间存在传播,这有必要采取公共卫生干预措施,以减少钩端螺旋体病在流行病学上的影响。地区。

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