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Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

机译：疑似马的马的寄生虫学，血清学和分子检测的比较诊断

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摘要

Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference (P>0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference (P<0.05) in the prevalence of the disease between emaciated and animals with good body condition. Assessment of the degree of agreement of the diagnostic tests employed revealed low to fair [graphic removed] with significantly higher sensitivity by PCR than other tests.
机译：对来自埃塞俄比亚两个Arsi-Bale高地可疑地区的237匹马进行了寄生虫学，血清学和分子学测试的比较敏感性研究，以确定该疾病的流行程度和诊断测试的一致性。因此，通过寄生虫学Woo试验，RoTat 1.2试验和18S PCR试验，发现该疾病的患病率分别为4.6％，36.7％和47.6％。该疾病的血清阳性率在CATT / Trypanosoma evansi试验中为27.6％。在埃塞俄比亚，使用Woo测试首次在4.6％的动物中证实了来自可疑马匹的锥虫。本研究的发现表明，杜鹃是非常普遍的，并且是该地区马的主要疾病之一。动物的地区，性别和年龄组之间的疾病患病率无统计学差异（P> 0.05）。但是，消瘦的和身体状况良好的动物之间的疾病发生率有统计学差异（P <0.05）。对使用的诊断测试的一致程度的评估显示，PCR灵敏度比其他测试低至中等（已去除图形），并且灵敏度明显更高。

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