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首页> 外文期刊>Transplantation: Official Journal of the Transplantation Society >Susceptibility of chacma baboons (Papio ursinus orientalis) to infection by hepatitis B virus (see comments)
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Susceptibility of chacma baboons (Papio ursinus orientalis) to infection by hepatitis B virus (see comments)

机译：狒狒（东方狒狒）易感染乙型肝炎病毒（见评论）

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BACKGROUND: Because baboons are being considered as a source of xenografts for human liver transplantation in patients with hepatitis B virus- (HBV) induced cirrhosis to forestall infection of the graft by the virus, we undertook a study to ascertain if baboons are resistant to HBV infection. METHODS: Six chacma baboons were inoculated with serum containing HBV and were followed for 52 weeks to detect transmission of infection. RESULTS: Anti-HBc was detected in the serum of four baboons 16 weeks after inoculation. Virions, small spherical particles, and tubular forms were seen at this time in the serum of the one baboon studied by transmission electron microscopy. HBV DNA was detected by polymerase chain reaction in the serum of the same four baboons throughout the period of follow-up, as well as in liver tissue obtained after 52 weeks. The specificity of the DNA was confirmed by Southern hybridization. Nucleotide sequences showed complete sequence identity between the HBV DNA in each of the baboon sera and one of the two HBV genotypes inoculated. Serum transaminase levels tested at 4-weekly intervals were always normal and histological examination of liver tissue after 52 weeks showed no evidence of chronic hepatitis. Examination of squash preparations of liver tissue by electron microscopy in one baboon revealed core-like particles. CONCLUSIONS: Chacma baboons are susceptible to HBV infection and appear to develop a chronic carrier state. The use of xenografts from baboons should preferably be avoided, but if they are used again for HBV-infected patients it would be prudent to treat the patients as if they had received an organ from a human donor.
机译：背景：由于狒狒被认为是乙型肝炎病毒（HBV）引起的肝硬化患者的人类肝移植的异种移植物，从而阻止了该病毒对移植物的感染，因此我们进行了一项研究，以确定狒狒是否对HBV耐药感染。方法：将六只狒狒狒狒接种含HBV的血清，并随访52周以检测感染的传播。结果：接种16周后，在四个狒狒的血清中检测到抗HBc。通过透射电子显微镜研究，此时在一只狒狒的血清中可见病毒颗粒，小的球形颗粒和管状形式。在整个随访期间，通过聚合酶链反应在相同的四个狒狒的血清中以及在52周后获得的肝组织中检测到HBV DNA。通过Southern杂交证实了DNA的特异性。核苷酸序列显示出每个狒狒血清中的HBV DNA与所接种的两种HBV基因型之一之间具有完全的序列同一性。每隔4周检测一次血清转氨酶水平始终正常，52周后肝组织的组织学检查未显示出慢性肝炎的迹象。在一只狒狒中通过电子显微镜检查肝脏组织的壁球制剂显示出核样颗粒。结论：Chacma狒狒容易感染HBV，并且似乎发展为慢性携带者状态。最好避免使用狒狒的异种移植物，但如果将它们再次用于HBV感染的患者，则应谨慎对待患者，就像他们已经从人类供体中获得器官一样。

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