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首页> 外文期刊>Transplantation: Official Journal of the Transplantation Society >Use of primary human liver cells originating from discarded grafts in a bioreactor for liver support therapy and the prospects of culturing adult liver stem cells in bioreactors: a morphologic study.
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Use of primary human liver cells originating from discarded grafts in a bioreactor for liver support therapy and the prospects of culturing adult liver stem cells in bioreactors: a morphologic study.

机译：来自废弃反应器的原代人类肝细胞在生物反应器中用于肝支持治疗的用途以及在生物反应器中培养成年肝干细胞的前景：一项形态学研究。

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INTRODUCTION: The development of a bioreactor providing a three-dimensional network of interwoven capillary membranes with integrated oxygenation and decentralized mass exchange enables the culture of primary human liver cells from discarded donor organs for extracorporeal liver support. METHODS: Primary liver cells were isolated from 54 discarded organs (donor age 56.7+/-13.2 years). Between 2.8x10(10) and 6.4x10(10) parenchymal cells (PC) were cocultured with nonparenchymal cells (NPC) of the same organ in bioreactors (n=36). The metabolic activity of the cells was regularly determined during culture. The cell morphology and ultrastructure were investigated after culture periods of 1 to 5 weeks. RESULTS: Cell metabolism was maintained over at least 3 weeks after a phase of adaptation lasting 2 to 3 days. Through the use of transmission electron microscopy and immunohistochemistry, it was demonstrated that PC and NPC spontaneously formed tissue-like structures. Vascular cavities (CD 31 immunoreactivity [IR]) and bile duct-like channels (CK 19 IR), both exhibiting proliferation activity (Ki-67 IR), were regularly distributed. Some of the bile duct-like channels showed similarities to the Canals of Hering found in the natural liver. Cells expressing morphologic and antigenic characteristics of adult liver stem cells (CD 34 IR and c-kit IR) and areas with cells that showed both hepatocyte and biliary characteristics were detected. CONCLUSION: The results show that primary human liver cells obtained from discarded donor organs recover and can be maintained in bioreactors for clinical liver support therapy. In addition, initial observations on adult liver stem-cell culture in bioreactors are presented.
机译：简介：生物反应器的开发提供了一个三维交织的毛细血管网网络，具有集成的氧合作用和分散的质量交换功能，可培养来自废弃供体器官的原代人肝细胞，以进行体外肝支持。方法：从54个废弃器官（供体年龄56.7 +/- 13.2岁）中分离原代肝细胞。在生物反应器中，将2.8x10（10）和6.4x10（10）之间的实质细胞（PC）与同一器官的非实质细胞（NPC）共培养（n = 36）。在培养过程中定期测定细胞的代谢活性。培养1至5周后研究细胞形态和超微结构。结果：适应期持续2至3天后，细胞代谢至少维持3周。通过使用透射电子显微镜和免疫组织化学，证明了PC和NPC自发形成组织样结构。均表现出增殖活性（Ki-67 IR）的血管腔（CD 31免疫反应[IR]）和胆管样通道（CK 19 IR）定期分布。一些类似于胆管的通道显示出与天然肝中发现的黑灵运河相似。检测到表达成年肝干细胞的形态学和抗原学特征的细胞（CD 34 IR和c-kit IR），以及具有显示肝细胞和胆汁特征的细胞区域。结论：结果表明，从废弃的供体器官获得的原代人肝细胞可以恢复并可以保存在生物反应器中进行临床肝支持治疗。另外，提出了关于在生物反应器中成人肝干细胞培养的初步观察。

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