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首页> 外文期刊>Turkish Journal of Fisheries & Aquatic Sciences >Immunohistochemical and partial characterization studies of carbonic anhydrase in the brain of teleost fish Cyprinus carpio.
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Immunohistochemical and partial characterization studies of carbonic anhydrase in the brain of teleost fish Cyprinus carpio.

机译：硬骨鱼鲤鱼脑中碳酸酐酶的免疫组织化学和部分表征研究。

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摘要

Carbonic anhydrase (CA) activity is related to a variety of intermingled biological processes, including respiration, ionic transport, acid-base balance, and calcification. Carps Cyprinus carpio were investigated with respect to purification, partial characterization, and immunohistochemical localization of brain CA. The enzyme was purified up to 72 fold by affinity chromatography on sulfanilamide sepharose gel. The result is a yield of 59.6% and a specific activity of 1143 EU/mg protein with an apparent molecular weight of 28.6 kDa. The optimum pH and temperature were observed at 8.0 and 35 degrees C, respectively. Ions of Na+, K+, Mg2+, and Ca2+ at concentrations of 1, 5, and 10 mM exhibited up to 25% to 30% inhibition on brain CA activity, whereas Cl- exhibited the most inhibition on enzyme activity. Acetazolamide also displayed significant inhibition on brain CA activity (54%, 83%, and 92% at respective concentrations of 1, 5, and 10 mM). Antibodies against purified gill CA from trout Oncorhynchus mykiss reacted positively only to one band corresponding to CA in carp brain supernatant. Immunohistochemical staining was observed in oligodendrocytes, its processes, and in myelinated fibers. Our findings are similar to those of previous studies on higher vertebrates CA II.
机译：碳酸酐酶（CA）的活性与各种混杂的生物过程有关，包括呼吸，离子迁移，酸碱平衡和钙化。对鲤鱼鲤进行了关于脑CA的纯化，部分表征和免疫组化定位的研究。通过在磺酰胺琼脂糖凝胶上的亲和色谱将酶纯化至72倍。结果是产率为59.6％，比活性为1143EU / mg蛋白，表观分子量为28.6kDa。最佳pH和温度分别在8.0和35摄氏度下观察到。浓度为1、5的Na + ，K + ，Mg 2 + 和Ca 2 + 的离子，而10 mM对大脑CA活性的抑制作用最高达25％至30％，而Cl -对酶活性的抑制作用最大。乙酰唑胺还显示出对脑CA活性的显着抑制（分别在1、5和10 mM浓度下分别为54％，83％和92％）。来自鳟鱼Onmyhynchus mykiss的纯化gCA抗体仅对鲤鱼脑上清液中对应于CA的一条带发生阳性反应。在少突胶质细胞，其过程和有髓纤维中观察到免疫组织化学染色。我们的发现与先前对高等脊椎动物CA II的研究相似。

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