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首页> 外文期刊>Turkish Journal of Fisheries & Aquatic Sciences >Molecular cloning and expression analysis of extra sex combs gene during development in Macrobrachium nipponense.
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Molecular cloning and expression analysis of extra sex combs gene during development in Macrobrachium nipponense.

机译：日本沼虾发育过程中额外性梳基因的分子克隆和表达分析。

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摘要

The extra sex combs (Esc) gene first identified in Drosophia encodes a transcriptional repressor of homeotic genes belonging to the polycomb group (PcG) gene and is primarily expressed in the early embryos. In this study, we have isolated the full-length cDNA of an Esc gene from the testis of oriental river prawn (Macmbrachium nipponense) according to the established expressed sequence tags (ESTs) information using Rapid Amplification of the cDNA Ends (RACE) technique, designated as MnEsc. The full-length cDNA of MnEsc is 1,461bp in size and has an open reading frame (ORF) of 1,068bp, encoding a 355-amino acid protein. Real-time quantitative PCR analyses revealed that the expression level of MnEsc varied significantly in the developing embryo, postembryonic larval and adult tissues. Real-time quantitative PCR showed the MnEsc gene was expressed in testis, liver, ovary, brain, abdominal ganglion, heart, intestine and muscle with the highest level of expression in ovary and brain. In vivo silencing of the gene, the dsRNA of MnEsc caused a significantly decrease in target gene expression level in brain and ovary tissues, but no exterior appearance change of experimental shrimp was observed.
机译：首先在果蝇中鉴定出的额外性梳（Esc）基因编码属于polycomb group（PcG）基因的同源基因的转录阻遏物，主要在早期胚胎中表达。在这项研究中，我们使用cDNA末端快速扩增（RACE）技术根据已建立的表达序列标签（ESTs）信息，从东方河虾（Macmbrachium nipponense）的睾丸中分离了Esc基因的全长cDNA，指定为MnEsc。 MnEsc的全长cDNA大小为1,461bp，开放阅读框（ORF）为1,068bp，编码355个氨基酸的蛋白质。实时定量PCR分析显示，MnEsc的表达水平在发育中的胚胎，胚胎后幼虫和成年组织中有显着差异。实时定量PCR显示，MnEsc基因在睾丸，肝脏，卵巢，脑，腹部神经节，心脏，肠和肌肉中表达，在卵巢和脑中表达最高。在体内沉默该基因时，MnEsc的dsRNA导致脑和卵巢组织中靶基因表达水平显着降低，但未观察到实验虾的外观变化。

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