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首页> 外文期刊>Turkish Journal of Fisheries & Aquatic Sciences >Comparative Analysis of Using Isozyme and Issr-Pcr Markers for Population Differentiation of Cyprinid Fish
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Comparative Analysis of Using Isozyme and Issr-Pcr Markers for Population Differentiation of Cyprinid Fish

机译：使用同工酶和Issr-Pcr标记进行鲤鱼种群分化的比较分析

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摘要

The article presents data on genetic variability and differentiation of three species of cyprinid fish - roach Rutilus rutilus, ide Leuciscus idus and dace Leuciscus leuciscus baikalensis in the rivers of Siberia, Russia. From the three species studied ide has the lowest indices of genetic variability and more pronounced population differentiation. About 40% of isozyme loci are polymorphic in this species and the same percent - for nuclear DNA inter-simple sequence repeat (ISSR) markers; interpopulation component accounts for 62% of genetic variability. In roach indicators of genetic variability are higher, especially for DNA markers (67%), and interpopulation differentiation is less pronounced (FST = 0.550; GST = 0.214). Dace has the most significant genetic diversity and low differentiation of populations. 48% of the isozyme loci and 83% of multilocus DNA markers are polymorphic in this species, the interpopulation component accounts for 27% and 19% for two types of markers, respectively. In comparison with allozymes, ISSR markers provide higher estimates of genetic distances while making intrageneric comparisons and similar or somewhat lower values - while making a comparison of different groups. Isozyme markers provide more precise geographical differences of population groups of fish.
机译：本文介绍了在俄罗斯西伯利亚河中的三种鲤科鱼类的遗传变异和分化数据，这些鱼类是蟑螂，红util，白斑鱼和白斑鱼。在所研究的三个物种中，ide具有最低的遗传变异指数和更显着的种群分化。在该物种中，约40％的同工酶基因座是多态性的，并且相同的百分比-对于核DNA间简单序列重复（ISSR）标记;种群间的成分占遗传变异的62％。在蟑螂中，遗传变异性的指标较高，尤其是DNA标记（67％），种群间的分化不太明显（FST = 0.550; GST = 0.214）。戴斯（Dace）具有最重要的遗传多样性，且种群分化程度低。该物种中48％的同工酶基因座和83％的多基因座DNA标记是多态性的，种群间的成分分别占两种标记的27％和19％。与同工酶相比，ISSR标记在进行内部比较时可提供更高的遗传距离估计，而在比较不同组时则可提供相似或略低的值。同工酶标记提供了鱼类种群的更精确的地理差异。

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