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Assessment of pineapple plants developed from micropropagation instead of conventional suckering

机译：通过微繁殖而不是传统的抽油对菠萝植物进行评估

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Rapid in vitro multiplication of pineapple by culture establishment, shoot multiplication and rooting of plantlets has been developed. Murashige and Skoog (MS) medium containing 50 mg/l adenine hemisulphite, 3 percent sucrose, 10 percent coconut water, 2 mg/l naphthalene acetic acid (NAA) and 0.5 mg/l 6-benzyl amino purine (BAP) gave the most (76 percent) axenic bud cultures which sprouted within 20-25 days. MS with 3.2 mg/l BAP and 0.25 mg/l NAA gave most lateral shoots per month at the 4th stage, which was found to decline to 4. I thereafter. Tufts of shoots when separated into indivIduals rooted best on half strength MS with 2 percent sucrose and 1 mg/l each of indole butyric acid and NAA. Around 88 percent of the rooted plants became established in the field, where micropropagated plants performed as well as conventional suckers with no somaclonal variation. The micropropagated plantlets took more time to flowering and harvesting but they flowered well. The quality of the fruits from the micropropagated plants was comparable with those from suckers.
机译：已经开发了通过培养建立，菠萝繁殖繁殖和幼苗生根快速菠萝体外繁殖的方法。含50 mg / l腺嘌呤半亚硫酸盐，3％蔗糖，10％椰子水，2 mg / l萘乙酸（NAA）和0.5 mg / l 6-苄基氨基嘌呤（BAP）的Murashige和Skoog（MS）培养基效果最佳（76％）在20至25天内发芽的树苗芽培养物。 MS在第4阶段每月有3.2 mg / l的BAP和0.25 mg / l的NAA产生每月最多的侧生芽，之后下降到4。当成束的个体分开时，茎的簇生最好在半强度MS上进行，MS用2％的蔗糖和吲哚丁酸和NAA各1 mg / l。大约88％的生根植物已在田间建立，微繁殖植物的表现与常规抽油机一样，无体细胞变异。微繁殖的苗花更多的时间来开花和收获，但它们开花良好。微繁植物的果实品质与抽油烟果实相当。

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