A rapid bioassay for screening yam germplasm for response to anthracnose

K. R. Green; M. M. Abang; C. Iloba

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A rapid bioassay for screening yam germplasm for response to anthracnose

机译：用于筛选山药种质对炭疽病反应的快速生物测定

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A rapid detached leaf bioassay has been developed for routine screening of yam germplasm (Dioscorea spp.) for response to anthracnose (Colletotrichum gloeosporioides). Five single spore isolates of C. gloeosporioides representing three virulence groupings were used to screen five D. alata cultivars with reportedly varying host reactions. There were significant main effects in disease severity due to cultivar, isolate and a significant cultivar X isolate interaction, indicating that the assay differentiates isolate virulence and host reaction to anthracnose. A whole-plant assay showed a significant correlation with the detached leaf technique, confirming that the latter was an accurate indicator of cultivar susceptibility. The bioassay uses little inoculum and space and can be carried out under controlled conditions with selected isolates.

机译：已经开发出一种快速分离的叶片生物测定法，用于常规筛选山药种质（Dioscorea spp。），以响应炭疽病（Colletotrichum gloeosporioides）。五个代表三个毒力分组的球孢梭菌的五个单孢子分离物用于筛选五个D. alata品种，据报道它们具有不同的宿主反应。由于品种，分离株和显着的品种X分离株之间的相互作用，在疾病严重程度方面有重要的主要影响，这表明该分析方法可区分分离株的毒力和宿主对炭疽病的反应。整株植物测定显示与离叶技术有显着相关性，证实了离叶技术是栽培品种敏感性的准确指示。该生物测定法几乎没有接种物和空间，并且可以在控制条件下使用选定的分离物进行。

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《Tropical Science》 |2000年第3期|共7页
作者
K. R. Green; M. M. Abang; C. Iloba;
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正文语种 eng
中图分类农作物;
关键词
yam; anthracnose; Colletotrichum gloeosporioides; bioassay; resistance;

机译：山药;炭疽病;炭疽病;生物测定;抗性;

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专利

1. A rapid bioassay for screening yam germplasm for response to anthracnose [J] . K. R. Green, M. M. Abang, C. Iloba Tropical Science . 2000,第3期

机译：用于筛选山药种质对炭疽病反应的快速生物测定
2. Rapid assessment of resistance of tissue-cultured water yam (Dioscorea alata) and white guinea yam (Dioscorea rotundata) to anthracnose (Colletotrichum gloeosporioides Penz.). [J] . Popoola A. R., Adedibu B. O., Ganiyu S. A. Archives of Phytopathology and Plant Protection . 2013,第5a8期

机译：快速评估组织培养的水山药（Dioscorea alata）和白色豚鼠山药（Dioscorea rotundata）对炭疽病（Colletotrichum gloeosporioides Penz。）的抗性。
3. Polymerase Chain Reaction Assay for Rapid, Sensitive Detection, and Identification of Colletotrichum gloeosporioides causing Greater Yam Anthracnose [J] . Mithun Raj, M. L. Jeeva, V. Hegde, Molecular Biotechnology . 2012,第3期

机译：快速，灵敏的检测和鉴定引起大山药炭疽病的炭疽菌炭疽病的聚合酶链反应分析
4. RAPID SCREENING OF WIIEAT GERMPLASM FOR FUSARIUM HEAD BLIGHT RESISTANCE. [C] . Martha Diaz de Ackermann International Symposium on Wheat Improvement for Scab Resistance May 5-11, 2000, Suzhou and Nanjing, China . 2000

机译：小麦胚芽的快速筛选，用于镰刀菌头部抗枯萎病。
5. Strawberry Studies: Screening of Germplasm and Identification of Quantitative Trait Loci for Necrotrophic and Hemibiotrophic Resistance to Anthracnose Diseases, and Validation of a Set of SSR Fingerprinting Markers [D] . Chacón Jimenez, José Guillermo. 2020

机译：草莓研究：筛查种质和鉴定成分性状对蒽酮疾病的病例和血管营养性抗性的鉴定，以及一组SSR指纹标记的验证
6. A Robust Automated Image-Based Phenotyping Method for Rapid Vegetative Screening of Wheat Germplasm for Nitrogen Use Efficiency [O] . Giao N. Nguyen, Pankaj Maharjan, Lance Maphosa, 2019

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7. Screening of horsegram germplasm collected from Andhra Pradesh against anthracnose [O] . A. Udaya Sankar, K. Anitha, N. Sivaraj, 2015

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8. Development of a Rapid, Inexpensive Bioassay for Screening Contaminant Bioavailability in Sediment Using mRNA Profiling [R] . Perkins, E. , Frederickson, H. L. , Lotufo, G. 2002

机译：开发一种快速，廉价的生物测定法，用于使用mRNa分析筛选沉积物中的污染物生物利用度

A rapid bioassay for screening yam germplasm for response to anthracnose

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