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首页> 外文期刊>Carbohydrate research >H-1 NMR spectroscopy study of the dynamic properties of glycogen in solution by steady-state magnetisation measurement with off-resonance irradiation
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H-1 NMR spectroscopy study of the dynamic properties of glycogen in solution by steady-state magnetisation measurement with off-resonance irradiation

机译：H-1 NMR光谱通过非共振辐射稳态磁化测量溶液中糖原的动态性质

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摘要

The dynamics of size-selected fractions of glycogen in solution have been investigated by proton NMR spectroscopy, using a recently described relaxation study method which relies on strong off-resonance irradiation. The dependence of the steady-state magnetisation on angle and intensity of the effective radio-frequency field was measured and compared to theoretical curves derived from different models of motion. Absence or presence of contributions to relaxation from molecular motions on the microsecond time scale can be tested with this method, without having to resort to models. We found that glycogen dipolar relaxation did not result from isotropic Brownian rotation, and despite some contribution from slow motion (>1 mu s) to relaxation in glycogen alpha-particles extracted from rat liver, bulk movement of the molecules did not appear to participate in averaging the dipolar term to zero. Whereas hepatic glycogen rat beta-particles and commercial oyster glycogen displayed very similar relaxation properties, alpha-particles showed significantly different behaviour. However, all results were compatible with a diversity of movements within the molecule, ranging from freely rotating pyranoside rings through collective chain motion and possibly to bulk movement of the beta sub-units within the alpha-particle. (C) 1998 Elsevier Science Ltd. All rights reserved. [References: 36]
机译：溶液中糖原的大小选择部分的动力学已通过质子核磁共振波谱法进行了研究，使用了最近描述的弛豫研究方法，该方法依赖于强共振共振辐射。测量了稳态磁化强度对有效射频场的角度和强度的依赖性，并将其与源自不同运动模型的理论曲线进行了比较。可以使用这种方法来测试是否存在或不存在微秒级尺度上的分子运动对弛豫的贡献，而无需借助模型。我们发现糖原偶极弛豫不是由各向同性布朗旋转引起的，尽管慢动作（> 1μs）对从大鼠肝脏中提取的糖原α颗粒的弛豫有一定贡献，但分子的整体运动似乎并未参与将偶极项平均为零。肝糖原大鼠β颗粒和商业牡蛎糖原显示出非常相似的弛豫特性，而α颗粒则表现出明显不同的行为。但是，所有结果均与分子内的多种运动兼容，从自由旋转的吡喃糖苷环通过集体链运动到可能与在α粒子内的β亚基整体运动无关。 （C）1998 Elsevier ScienceLtd。保留所有权利。 [参考：36]

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