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首页> 外文期刊>Veterinary Immunology and Immunopathology >Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics
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Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics

机译：通过分子克隆，测序和系统发育学比较评估沼泽型水牛和其他水牛品种的Th1和Th2细胞因子

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摘要

Comparative assessment of Th1 and Th2 cytokines of three bubaline breeds namely swamp buffalo, its crossbreed with riverine buffalo (CB), and the improved breed of Bulgarian Murrah buffalo (BMB), was done by molecular cloning, sequencing and phylogenetic analysis. The Th1 cytokines analyzed included IL-2, IL-12p35, IL-12p40, and IFN-gamma while Th2 cytokines included IL-4 and IL-10. Both groups showed strict conservation in the putative secondary structures and amino acid residues within the tribe Bovini, which indicated functional cross-reactivity. Nucleotide sequence homology ranged from 98.6 to 100.0% and was lowest for IL-12p35. With regard to amino acid sequence, the lowest homology was observed in IL-4 with 97.8%. This substitution was mainly due to differences in mRNA splicing. The phylogenetic relationship of the buffalo breeds was analyzed and showed them as a cluster comprised mainly of species belonging to the order Artiodactyla, including cattle and pigs. A deeper knowledge of these cytokine structures will favor understanding of water buffalo immunology and how much it differs from its closest subspecies and other animals.
机译：通过分子克隆，测序和系统发育分析，对三种水牛品种（沼泽水牛，其与河水牛（CB）的杂种以及改良的保加利亚穆拉水牛（BMB））的Th1和Th2细胞因子进行了比较评估。分析的Th1细胞因子包括IL-2，IL-12p35，IL-12p40和IFN-γ，而Th2细胞因子包括IL-4和IL-10。两组均显示出Bovini部落中假定的二级结构和氨基酸残基的严格保守性，表明功能性交叉反应性。核苷酸序列同源性在98.6％至100.0％之间，对于IL-12p35最低。关于氨基酸序列，在IL-4中观察到最低的同源性，为97.8％。该取代主要是由于mRNA剪接的差异。分析了水牛品种的系统发育关系，并将它们显示为一个簇，主要由属于节肢动物的物种组成，包括牛和猪。对这些细胞因子结构的更深入的了解将有助于了解水牛的免疫学及其与最近的亚种和其他动物的差异。

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