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首页> 外文期刊>Veterinary Immunology and Immunopathology >Modulation of rainbow trout (Oncorhynchus mykiss) intestinal immune geneTI Modulation of rainbow trout (Oncorhynchus mykiss) intestinal immune gene expression following bacterial challenge
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Modulation of rainbow trout (Oncorhynchus mykiss) intestinal immune geneTI Modulation of rainbow trout (Oncorhynchus mykiss) intestinal immune gene expression following bacterial challenge

机译：虹鳟鱼（Oncorhynchus mykiss）肠道免疫基因的调节细菌攻击后虹鳟（Oncorhynchus mykiss）肠道免疫基因的表达调节

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The mucosal immune systems of fishes are still poorly understood, and defined models for studying natural host-pathogen interactions are lacking. The objective of this study was to evaluate different challenge models and pathogens to examine the magnitude of change in intestinal immune gene expression. Rainbow trout were exposed by immersion to Yersinia ruckeri or by intraperitoneal injection with Flavobacterium psychrophilum. At 3, 9, or 10 days post-challenge, pathogen load was quantified by plate count and intestinal tissue was removed and immune gene expression measured by real-time PCR. In general, the magnitude of infection was correlated with change in immune gene transcript abundance. We found that messages for the innate immune molecules, SAA, IL-8, INF-gamma and TNF-alpha, as well as the message for IgM, were up-regulated in intestinal tissue in both challenge paradigms. A >250-fold increase was observed in SAA and 20-fold increase of IL-8 gene transcript abundance occurred on day 10 following challenge with F. psychrophilum. Within individual fish, there was a positive correlation between bacteria load in the spleen and the increase of immune gene message between 3 and 10 days post-infection. These findings demonstrate that measurable changes in immune gene expression occur in the intestine of rainbow trout following bath challenge with Y. ruckeri or injection challenge with F. psychrophilum
机译：鱼类的粘膜免疫系统仍知之甚少，缺乏用于研究自然宿主与病原体相互作用的明确模型。这项研究的目的是评估不同的挑战模型和病原体，以检查肠道免疫基因表达变化的幅度。虹鳟鱼通过浸入ruckeria ruckeri或腹膜内注射Psychoophilum进行暴露。攻击后3、9或10天，通过板数对病原体负荷进行定量，并去除肠组织，并通过实时PCR测量免疫基因表达。通常，感染的程度与免疫基因转录物丰度的变化相关。我们发现先天免疫分子，SAA，IL-8，INF-γ和TNF-alpha的信息以及IgM的信息在两种挑战范式中的肠组织中均上调。在用F. psychrophilum攻击后第10天，SAA观察到> 250倍的增加，IL-8基因转录丰度增加了20倍。在个别鱼类中，感染后3至10天之间，脾脏中的细菌负荷与免疫基因信息的增加之间存在正相关。这些发现表明，在用Y. ruckeri进行浴攻击或用F. psychrophilum进行注射攻击后，虹鳟鱼的肠道中免疫基因表达发生了可测量的变化。

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