...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Veterinary Immunology and Immunopathology >CD45+and CD45-lymphocyte populations identified by flow cytometry from dogs with lymphoma exhibit similar morphology and the same clonal (B cell or T cell) lineage
【24h】

CD45+and CD45-lymphocyte populations identified by flow cytometry from dogs with lymphoma exhibit similar morphology and the same clonal (B cell or T cell) lineage

机译：通过流式细胞术从患有淋巴瘤的狗中鉴定出的CD45 +和CD45-淋巴细胞群具有相似的形态和相同的克隆（B细胞或T细胞）谱系

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Flow cytometric analysis of canine lymphoma sometimes demonstrates a mixed population of CD45+ and CD45- lymphocytes. Recently, indolent forms of canine lymphoma have been described which are associated with the loss of CD45 expression, warranting further investigation of the role of CD45 in canine lymphoma. The purpose of this study was to compare morphology and assess clonal origin between CD45+ and CD45- lymphocyte populations identified by flow cytometry in confirmed cases of canine B- and T-cell lymphoma. Our hypothesis was that the CD45- population of lymphocytes represented a phenotypic variant of the CD45+ population. Fifteen client-owned dogs with lymphoma and distinct CD45+ and CD45- lymphocyte populations identified by flow cytometry were identified for a blinded, prospective assessment of morphology and clonal origin (B cell or T cell) between populations of sorted CD45+ and CD45- cells. Lymphocytes were isolated from 11 dogs for paired cytologic evaluation. In 10/11 dogs, the CD45+ and CD45- samples were similar (95% C.I., 0.301-1.00). DNA was harvested from sorted populations of CD45+ and CD45- cells from 12/15 dogs and PARR analysis produced amplicons of identical size from both populations, indicating that 100% (12/12) were of the same lineage, B cell or T cell (95% Cl., 0.757-1.00). Collectively, our data suggests that the CD45- population identified in dogs with lymphoma represents a phenotypic variant of the CD45+ population. (C) 2015 Elsevier B.V. All rights reserved.
机译：犬淋巴瘤的流式细胞术分析有时显示出CD45 +和CD45-淋巴细胞混合存在。最近，已经描述了惰性形式的犬淋巴瘤，其与CD45表达的丧失有关，需要进一步研究CD45在犬淋巴瘤中的作用。本研究的目的是比较流式细胞术鉴定的犬B细胞和T细胞淋巴瘤病例中CD45 +和CD45-淋巴细胞群的形态，并评估其克隆起源。我们的假设是淋巴细胞的CD45-群体代表CD45 +群体的表型变异。通过流式细胞术鉴定了15只客户拥有的淋巴瘤和不同的CD45 +和CD45-淋巴细胞群的狗，以对分类的CD45 +和CD45-细胞群之间的形态和克隆起源（B细胞或T细胞）进行盲目，前瞻性评估。从11只狗中分离出淋巴细胞用于配对细胞学评估。在10/11犬中，CD45 +和CD45-样品相似（95％C.I.，0.301-1.00）。从12/15只狗的CD45 +和CD45-细胞分选群体中收集DNA，并且PARR分析从这两个群体中产生大小相同的扩增子，表明100％（12/12）具有相同的谱系，B细胞或T细胞（ 95％Cl。，0.757-1.00）。总体而言，我们的数据表明，在患有淋巴瘤的狗中鉴定出的CD45-群体代表了CD45 +群体的表型变异。 （C）2015 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号