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首页> 外文期刊>Veterinary Dermatology >Altered mRNA and protein expression of filaggrin in the skin of a canine animal model for atopic dermatitis
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Altered mRNA and protein expression of filaggrin in the skin of a canine animal model for atopic dermatitis

机译：犬异位性皮炎动物模型皮肤中丝聚蛋白的mRNA和蛋白表达改变

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Background - Filaggrin is a structural protein that has attracted increasing interest over the past decade for its role in the pathogenesis of human atopic dermatitis (AD). Null mutations in its sequence are considered risk factors in the development of AD. Hypothesis/Objectives - To investigate canine filaggrin mRNA and protein expression in the skin of atopic beagles with experimentally induced AD compared with breed-matched healthy control dogs. Methods - All dogs were environmentally challenged for 3 days consecutively with allergens to which the atopic dogs had been sensitized. Skin biopsy specimens were taken from six healthy and seven atopic beagles before and after allergen challenge. Canine filaggrin mRNA was measured using quantitative real-time PCR. Indirect immunofluorescence was used to localize the filaggrin protein in canine skin. Analysis of variance with Tukey's multiple comparison test (over-time effect) and unpaired Student's t-test (treatment effect) were used. Values of P <= 0.05 were considered significant. Results - Analysis of variance showed a significantly higher expression of filaggrin mRNA in atopic dogs compared with healthy control dogs (P = 0.004 on day 3 and P = 0.01 on day 10) and a decreased mRNA expression on day 3 in healthy control dogs (effect of time, P = 0.006). On blinded evaluation, filaggrin immunofluorescence was distributed homogeneously in the stratum granulosum and the stratum corneum in healthy dogs. Atopic dogs showed a patchy immunofluorescence pattern, which was exacerbated after environmental challenge. Conclusions and clinical importance - Altered epidermal filaggrin mRNA expression and protein distribution was detected in this experimental model.
机译：背景-聚精蛋白是一种结构蛋白，由于其在人类特应性皮炎（AD）发病机理中的作用，在过去十年中引起了越来越多的关注。其序列中的无效突变被认为是AD发展的危险因素。假设/目的-研究与实验动物配对的健康对照犬相比，实验性诱导AD的特应性小猎犬皮肤中犬丝蛋白的mRNA和蛋白表达。方法-所有犬均连续3天接受特应性犬致敏的变应原对环境的攻击。在过敏原攻击之前和之后，从六个健康的和七个异位的小猎犬中获取皮肤活检标本。用定量实时PCR测量犬丝蛋白mRNA。间接免疫荧光用于定位犬皮肤中的丝聚蛋白。使用Tukey的多重比较检验（超时效应）和未配对的Student t检验（治疗效应）进行方差分析。 P <= 0.05的值被认为是显着的。结果-方差分析显示，特应性犬中丝聚蛋白mRNA的表达明显高于健康对照犬（第3天P = 0.004，第10天P = 0.01），而健康对照犬第3天mRNA表达降低（效果时间P = 0.006）。通过盲法评价，健康犬的颗粒蛋白层和角质层中丝聚蛋白免疫荧光均匀分布。特应性狗表现出斑驳的免疫荧光模式，在环境挑战后加剧。结论和临床重要性-在该实验模型中检测到表皮丝聚蛋白mRNA表达和蛋白分布改变。

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